한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권6호
  • /
  • Pages.657-663
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

pKT230 벡터를 이용한 Pseudomonas sp. P20의 2,3-Dihydroxybiphenyl Dioxygenase 유전자의 클로닝

  • 김지영 (충북대학교 자연과학대학 미생물학과) ;
  • 김치경 (충북대학교 자연과학대학 미생물학과) ;
  • 가종억 (서울대학교 분자미생물학연구센터) ;
  • 민경희 (서울대학교 분자미생물학연구센터) ;
  • 박용근 (서울대학교 분자미생물학연구센터)
  • 발행 : 1996.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Pseudomonas sp. P20 isolated from the polluted environment is capable of degrading biphenyl and 4-chlorobiphenyl. The pcbABCD genes responsible for degradation of biphenyl and 4-chlorobiphenyl were cloned using pBluescript SK(+) from the chromosomal DNA of Pseudomonas sp. P20 to construct pCK1 and pCK102, harbouring pcbABCD and pcbCD, respectively. The 2, 3-DHBP dioxygenase gene, pcbC, was cloned again from pCK102 by using pKT230 which is known as a shuttle vector and pKK1 hybrid plasmid was constructed. The E. coli KK1 transformant obtained by transforming the pKK1 into E. coli XL1-Blue showed 2, 3-DHBP dioxygenase activity. The specific 2, 3-DHBP dioxygenase activity of E. coli KK1 was similar to that of the E. coli CK102, but much higher than those of the natural isolates, Pseudomonas sp. DJ-12 and Pseudomonas sp. P20.

키워드

참고문헌

  1. J. Bacteriol. v.175 Three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the Gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6 Asturias,J.A.;K.N.Timmis
  2. Appl. Environ. Microbiol. v.61 Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. Masai,E.;A.Yamada;J.M.Healy;T.Hatta;K.Kimbara;M.Fukuda;K.Yano
  3. J. Bacteriol. v.172 Pseudomonas putida KF715 bphABCD operon encoding biphenyl and polychlorinated biphenyl degradation: cloning analysis and expression in soil bacteria Hayase,N.;K.Taira;K.Furukawa
  4. J. Bacteriol. v.176 Nucleotide sequence and functional analysis of the meta-cleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102 Kikuchi,Y.;Y.Yasukochi;Y.Nagata;M.Fukuda;M.Takagi
  5. Kor. J. Microbiol. v.30 Divergence of the cbp genes in 4-chlorobiphenyl catabolizing bacteria Youn,D.J.;J.J.Han;C.K.Kim;Y.Kim
  6. Appl. Environ. Microbiol. v.61 Degradation of polychlorinated biphenyl mixtures (Aroclors 1242, 1254 and 1260) by the white rotfungus Phanerochaete chrysosporium as evidenced by congener-specific analysis Yadav,J.S.;J.F.Quensen Ⅲ;J.M.Tiedje;C.A.Reddy
  7. J. Bacteriol. v.166 Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes Furukawa,K.;T.Miyazaki
  8. Kor. J. Microbiol. v.31 Cloning and expression of pcbAB genes from Pseudomonas sp. DJ-12 in Escherichia coli. Han,J.J.;T.K.Sung;C.K.Kim
  9. Appl. Environ. Microbiol. v.57 Expression, localization, and functional analysis of polychlorinated biphenyldegradation genes cbpABCD of Pseudomonas putida Khan,A.A.;S.K.Walia
  10. J. Bacteriol. v.171 Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation Mondello,F.J.
  11. FEMS Microbiol. Lett. v.113 A DNA module encoding bph genes for the degradation of polychlorinated biphenyls (PCBs) Dowling,D.N.;R.Pipke;D.F.Dwyer
  12. Appl. Environ. Microbiol. v.61 Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Peudomonas sp. strain LB400 Seeger,M.;K.N.Timmis;B.Hofer
  13. J. Bacteriol. v.171 Molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: Some soil bacteria possess a highly conserved bph operon Furukawa,K.;N.Hayase;K.Taira;N.Tomizuka
  14. Gene v.130 Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation Hofer,B.;L.D.Eltis;D.N.Dowling;K.N.Timmis
  15. Appl. Environ. Microbiol. v.62 Genetic structure of the genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoicacid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading Pseudomonas sp. strain DJ-12 Kim,E.;Y.Kim;C.K.Kim
  16. Gene v.125 Cloning and expression of the polychlorinated biphenyl-degradation gene cluster from Arthrobacter M5 and comparison to analogous genes from gram-negative bacteria Peloquin,L.;C.W.Greer
  17. J. Bacteriol. v.175 Oxidation of biphenyl by a multicomponent enzyme system from Pseudomonas sp. LB400 Haddock,J.D.;L.M.Nadim;D.T.Gibson
  18. FEMS Microbiol. Lett. v.118 Functional and structual relationship of various extradiol aromatic ring-cleavage dioxygenases of Pseudomonas origin Hirose,J.;N.Kimura;A.Suyama;A.Kobayashi;S.Hayashida;K.Furukawa
  19. Appl. Environ. Microbiol. v.38 Effect of chlorine substitution on the bacterial metabolism of various polychlorinated biphenyls Furukawa,K.;N.Tomizuka;A.Kamibayashi
  20. J. Bacteriol. v.177 Molecular and biochemical characterization of two meta-cleavage dioxygenases involved in biphenyl an m-xylene degradation by Beijerinckia sp. strain B1 Kim,E.;G.J.Zylstra
  21. J. Bacteriol. v.169 Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene Furukawa,K.;N.Arimura
  22. Appl. Environ. Microbiol. v.61 Multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the gram-positive polychlorinated biphenyl-degrading bacterium Rhodococcus erythropolis TA421, isolated from a termite ecosystem Maeda,M.;S.Chung;E.Song;T.Kudo
  23. J. Microbiol. Biotechnol. v.5 Cloning and expression of pcbC and pcbD genes responsible for 2,3-dihydroxybiphenyl degradation from Pseudomonas sp. P20 Nam,J.H.;H.M.Oh;C.K.Kim
  24. J. Bacteriol. v.171 Cloning and sequencing of two tandem genes involved in degradation of 2,3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS102 Kimbara,K.;T.Hashimoto;M.Fukuda;T.Koana;M.Takagi;M.Oishi;K.Yano
  25. FEMS Microbiol. Lett. v.106 Increased expression of the plasmid-determined 2,3-dihydroxybiphenyl dioxygenase gene in strains of Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa Andreyeva,A.L.;A.V.Slepenkyn;I.I.Starovoytov
  26. J. Bacteriol. v.133 RSF1010 plasmid as potentially useful vector in Pseudomonas species Nagahari,K.;K.Sakaguchi
  27. Gene v.16 Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas Bagdasarian,M.;R.Lurz;B.R.ckert;F.C.H.Franklin;M.M.Bagdasarian;J.Frey;K.N.Timmis
  28. Appl. Environ. Microbiol. v.43 Rapid screen for bacterial degrading water-insoluble, solid hydrocarbons on the agar plate Kiyohara,H.;K.Nagao;K.Yana
  29. A laboratory manual(2nd ed.) Molecular cloning Smbrook,J.;E.F.Fritsch;T.Maniatis
  30. Protein methods Bollag,D.M.;S.J.Edelstein
  31. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;N.J.Rosenbrought;N.E.Farr;R.J.Randall