Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

카라기난 분해효소 생산균의 분리, 동정 및 효소생산 최적 조건

  • 양승택 (경성대학교 식품공학과) ;
  • 주동식 (부경대학교 식품공학과) ;
  • 박중제 (부경대학교 식품공학과) ;
  • 이정석 (부경대학교 식품공학과) ;
  • 김명식 (부경대학교 식품공학과) ;
  • 이응호 (부경대학교 식품공학과)
  • Published : 1996.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The 80 strains which produce carrageenan degrading enzyme were isolated from soils, mud, seaweed, marine moluscus and echonodermata samples. Among them, one isolated strain, which showed the highest activity to produce carrageenan degrading enzyme, was used for this study. The isolated strain was identified as Pseudomonas alcaligenes through its morphological, biochemical, and physiological characteristics. The best conditions for enzyme production were 0.7% nutrient broth and 0.2% carrageenan as nitrogen and carbon source, respectively. The optimal pH, NaCl, temperature and culture time for carrageenan degrading enzyme were 7.0, 1.5%, 30* and 96hrs, respectively.

Keywords

References

  1. Carbohydrate and Carbohydrate Polymers De Clercq, E.;M.Yalpani(ed.)
  2. Food gels Stanley,N.F.;P.Harris(ed.)
  3. New Food Industry v.32 no.5 ケル化劑, 增粘劑としてのカラギ-ナンの特性とその商品への應用例について 岩瀨 弘士郞
  4. 海蘂の本 西澤一俊;村杉幸子
  5. New Food Industry v.28 no.4 カラギ-ナンとそのバイオリアクタ-への利用 小林賢一
  6. 化 學と生物 v.30 no.3 微生物による天然多糖からの有用オリゴ糖の生産 富田旁男;橫田 馬
  7. 月刊フドケミカル v.2 オリゴ糖の開發の現狀と展望 酒井重男
  8. Nippon Suisan Gakkaishi v.58 no.3 Isolation and some properties of alginate lyase from a marine bacterium Vibrio sp. AL-128 Tseng,C.H.;K.Yamauchi;M.Kitamikado
  9. 日本水産學會誌 v.29 no.2 アルギン酸から酵素分解によつて調劑したオリゴ糖の靜菌作用 北御門學;酉村光弘;山口邦子;會 照煌
  10. Can. J. Microbiol. v.23 Enzymatic hydrolysis of agar: purification and characterization of β-neoagarotetraose hydrolase from Pseudomonas atlantica Groleau,D.;W.Yaphe
  11. Eur. J. Biochem. v.137 β-Agarases Ⅰand Ⅱ from Pseudomonas atlantica : substrate specificities Morrice,L.M.;M.W.Mclean;W.F.Ling;F.B.Williamson
  12. Nippon suisan Gakkaishi v.58 no.8 Production of fucoidan degrading enzyme, fucoidanase, and fucoidan sulfatase by Vibrio sp. N-5 Furukaswa,S.;T.Fujikawa;D.Koga;A.Ide
  13. Biotech. Biochem. v.55 no.11 Purification and some properties of exo-type fucoidanase from Vibrio sp. N-5 Furukawa,S.;T.Fujikawa;D.Koga;A.Ide
  14. 韓國營養食糧學會誌 v.22 no.2 Vibrio sp. AI-145가 生産하는 菌體外酵素의 精製(Ⅰ) 周東植;李鷹昊
  15. 한국산업미생물학회지 v.23 no.4 알긴산 분해균 Vibrio sp. AI-145가 생산하는 균체내 효소의 정제 및 특성 주동식;이정석;박중제;조순영;안창범;이응호
  16. 한국식품과학회 v.28 no.1 효소분해에 의한 알긴산올리고당류의 제조 주동식;이정석;박중제;조순영;김희경;이응호
  17. J. Biol. Chem. v.195 Notes on sugar determination Somogyi,M.;N.Nelson
  18. Bergey's Manual of systematic Bacteriology v.1 Baumann,P.;A.L.Furniss;J.V.Lee
  19. Identification methods for microbiologist Gibbs,B.M.;F.A.Shinner
  20. J.of Ferm. & Bioengineering v.72 no.3 Bacterial alginate lyase; characterization of alginate lyasc producing bacteria and purification of the enzyme Yonemoto,Y.;K.Murata;A.Kimura;H.Yamaguchi;K.Okayama
  21. Can. J. Microbiol. v.5 An interpretation of the effects of salts on the lactic dehydrogenase of Halobacterium salinarium Baxter,R.M.