Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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이담자균 효모 Rhodosporidium toruloides에서 Rhodotorucine A에 의한 막단백질 인산화의 저해와 Trigger Peptidase의 관련성

  • 정영기 (동의대학교 미생물학과) ;
  • 이태호 (부산대학교 미생물학과) ;
  • 류병호 (경성대학교 식품공학과)
  • Published : 1996.12.01
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Abstract

[$\gamma$-$^{32}$P]ATP was used to test phosphorylation of membrane proteins of mating type a cells of heterobasidiomycetous yeast Rhodosporidium toruloides separated by non-denaturing electrophoresis. The phosphoprotein was observed in the membrane proteins. The phosphorylation was inhibited by the pheromone rhodotorucine A (Rh. A) secreted by mating type A of the yeast. Rh. A didn't inhibit the phosphorylation in the presence of a trigger peptidase (TPase) inhibitor, antipain. Partially digested Rh. A by trypsin maintained the phosphorylation inhibitory activity. These results show that TPase activity plays an important role in the transduction of pheromone signal in the yeast.

Keywords

References

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