Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Melittin-Hybrid 합성 펩타이드가 Fusarium oxysporum의 성장에 미치는 저해효과

  • 이동건 (한국과학기술연구원 생명공학연구소 펩타이드 공학 연구 그룹) ;
  • 신송엽 (한국과학기술연구원 생명공학연구소 펩타이드 공학 연구 그룹) ;
  • 이성구 (한국과학기술연구원 생명공학연구소 펩타이드 공학 연구 그룹) ;
  • 이명규 (한국과학기술연구원 생명공학연구소 펩타이드 공학 연구 그룹) ;
  • 함경수 (한국과학기술연구원 생명공학연구소 펩타이드 공학 연구 그룹)
  • Published : 1996.10.01
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Abstract

Melittin (ME) from honeybee venom has a broad range of strong antimicrobial activity, but it has hemolytic activity against eukaryotic cells. In order to design peptides with powerful antifungal activity without cytotoxic property of ME and understand structure-antifungal activity relationships, the hybrid peptides derived from the sequences of ME and cecropin A (CA) or magainin 2 (MA), MA(10-17)ME(1-12) and CA(1-8)ME(1-12). were synthesized by solid phase method. MA(10-17)ME(1-12) showed potent antifungal activity comparable to ME against Fusarium oxysporum with no hemolytic activity against human red blood cells. The hybrid peptides showed strong inhibi- tion of (1, 3)-$\beta$-D-glucan synthase. This result indicates that the antifungal activity of the hybrid peptides against Fusarium oxysporum is attributed to the inhibition of cell wall synthesis. The results therefore showed a successful design of a peptide having antifungal activity without hemolytic property.

Keywords

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