한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권4호
  • /
  • Pages.445-451
  • /
  • 1996
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Dicyma sp. YCH-37이 생산하는 효모세포벽 용해효소 I. 생산균주의 분리 및 효소의 정제

  • 정희철 (연세대학교 식품생물공학과 및 생물산업소재연구센터) ;
  • 함병권 (연세대학교 식품생물공학과 및 생물산업소재연구센터) ;
  • 배동훈 (단국대학교 식품공학과 및 연세대학교 생물산업소재연구센터) ;
  • 하세가와 토루 (일본 대판발효연구소) ;
  • 유주현 (연세대학교 식품생물공학과 및 생물산업소재연구센터)
  • 발행 : 1996.08.01
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초록

The strain YCH-37, which produces yeast cell wall lytlc enzyme, was isolated from soil. From the microscopic observation, morphological and cultural characteristics, this strain was identified to fungus, Dicyma sp. So, we named this strain as Dicyma sp. YCH-37. The lytic enzyme effectively lysed Salmonella typhimurium among intact living bacteria and Torulopsis, Hansenula, Zygosaccharomyces among intact living yeast, as well as autoclaved yeast strains. The yeast cell wall lytic enzyme was succesively purified to 204 folds with 13% yields through yeast glucan affinity adsorption and DEAE-cellulose column chromatography. The enzyme was identified to monomeric protein with molecular weight of 25,000 daltons from the results of SDS-PAGE and gel filtration. The optimum pH and temperature for the yeast lytic activity were 8.0 and 50$\circ$C, respectively. The enzyme was stable up to 40$\circ$C, and between pH 4.0-pH 10.0.

키워드

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