Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열

  • Published : 1996.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc$^{r}$) plasmid isolated from multi-drug resistant Staphylococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc$^{r}$ plasmid pTl8l. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only two base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.

Keywords

References

  1. Plasmid v.10 Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus Khan.S.A.;R.P.Novick
  2. Microbiol. Lett. v.5 Transformation of Bacillus subtilis with staphylococcal plasmid DNA Kono.M.;M.Sasatsu.;H.Hamashima
  3. J. Med. Microbiol. v.19 Antibiotic resistance in Staphylococcus aureus isolated at an Australian hospital between 1946 and 1981 Gillespie.M.T.;J.W.May.;R.A.Skurray
  4. 약학회지 v.39 다제내성 황색포도상구균이 가지고 있는 테트라사이클린 내성 플라스미드의 동정 이대운;문경호
  5. 약학회지 v.36 황색포도상구균에서 테트라사이클린 내성을 나타내는 플라스미드의 동정 김기현;김종명;문경호
  6. Molecular cloning Maniatis.T.;E.F.Fritsch.;J.Sambrook
  7. Molecular cloning Maniatis.T.;E.F.Fritsch.;J.Sambrook
  8. Proc. Natl. Acad Sci. v.74 DNA sequencing with chain-terminating inhibitors Sanger.F.;S.Nicklen.;A.R.Coulson
  9. J. Mol. Biol. v.215 Basis local alignment search tool Altschul.S.F.;W.Gish.;W.Miller.;E.W.Myers.;D.J.Lipman
  10. J. Biol. Chem. v.256 Unique features in the ribosome binding site sequencee of the Gram-positive Staphylococcus aureus β-lactamase gene McLaughlin.J.R.;C.L. Murray.;J.C.Rabinowittz
  11. J. Bacteriol. v.169 A site-specific recombination function in Staphylococcus aureus plasmids Gennaro.M.L.;J.Kornblum;R.P.Novick
  12. J. Bacteriol. v.170 Characterization of the tetracycline resistance genc of plasmid pT181 of Staphylococcus aureus Mojumdar.M.;S.A.Khan
  13. Plasmid v.30 The tet(K) gene of pT181 of Staphylococcus aureus encodes an efflux protein that contains 14 transmembrane helices Guay.G.G.;S.A.Khan.;D.M.Rothstein
  14. Gene v.21 Construction and propagation of deletion derivatives of staphylococcal tetracycline-resistance plasmid pTP5 inBacillus subtilis Noguchi.N.K.Shishido;T.Ando;M.Kono
  15. FEMS Microbiol. Lett. v.37 Determination of the complete nucleotide scquence of pNSI. a staphylococcal tetracycline-resistance plasmid propagated in Bacillus subtilis Noguchi.N.;T.Aoki.;M.Sasatsu.;M.Kono.;K.Shishido.;T.Ando
  16. J. Gen. Microbiol. v.132 Detection of an integrated tetracycline resistance plasmid in the chromosome of methicillin-resistant Staphylococcus aureus Gillespie.M.T.;J.W.May.;R.A.Skurray
  17. Mol. Gen Genet. v.195 Staphylococcal plasmid cointegrates are formed by host -and phage-mediated general rec systems that act on short regions of homology Novick.R.P.;S.J.Projan.;W.Rosenblum.;I.Edelman