Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cyanobacterium Synechocystis PCC6803 내에서 Bacillus thuringiensis sunsp. morrisoni PG-14 cryIVD 유전자의 발현

  • 이대원 (서울대학교 농생물학과) ;
  • 박현우 (서울대학교 농생물학과) ;
  • 진병래 (서울대학교 농생물학과) ;
  • 정영호 (한국표준과학연구원 기초과학지원연구소) ;
  • 박영목 (한국표준과학연구원 기초과학지원연구소) ;
  • 강석권 (서울대학교 농생물학과)
  • Published : 1996.04.01
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Abstract

Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASK5-1 was transformed into the cyanobacterium Syne- chocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of IVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that, the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

Keywords

References

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