Treatment of Wastewater Containing Phenol Using Pseudomonassp. B3

Pseudomonas sp. B3를 이용한 Phenol 함유 폐수의 처리

Using Pseudomonas sp. B3, identified and isolated from nature, wastewater containing phenol was treated in a continuous stirred tank reactor and its reaction characteristics were studied. Average concentrations of phenol and COD in effluents were 1.5mg/L and 124mg/L at 0.059h-1 dilution rate, respectively. At the dilution rate higher than 0.063h-1, phenol and COD increased abruptly to 19mg/L and 318mg/L. At the dilution rate higher than 0.059h-1, biomass concentration suddenly decreased and was "washed out". Biomass concentration was 150mg/L at a dilution rate of 0.067h-1. Maximum biomass production rate was 15.98mg/L$.$h at a dilution rate of 0.067h-1. When dilution rate increased above 0.059h-1, effluent phenol concentration abruptly increased and biomass production rate decreased. Maximum cell growth rate(${\mu}$max) and Michaelis-Mentens kinetic constant(Ks) were 0.074h-1 and 0.424mg/L, respectively. From the above result low phenol concentration can be expected at a maximum dilution rate, but reactor becomes unstable due to phenol inhibition.