KSBB Journal
- 제11권3호
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- Pages.288-294
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- 1996
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
혈청배지와 무혈청배지에서의 재조합 CHO 세포 성장과 Erythropoietin 생산
Characteristics of Recombinant CHO Cell Growth and Erythropoietin Production in Serum-Containing Media and Serum-Free Media
초록
CHO 세포를 0.5%, 1 %. 2%, 3%, 4% 및 5% 놓도의 FBSd를 함유한 MEMa 배지에
We have investigated the characteristics of recombinant CHO cell growth and erythropoletin(EPO) production at different concentrations of serum and inoculation density. Cell growth and EPO production were increased with the increase of serum concentration and inoculation density. Enhancement of CHO cell growth and EPO production by medium exchange using serum-free medium at the growth phase of cells was studied. It was found that the exchange of culture medium with serum-free medium was favorable for growth of cells and production of EPO. The maximum number of cell and concentration of EPO obtained by exchanging culture medium were
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