KSBB Journal
- Volume 11 Issue 1
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- Pages.8-14
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- 1996
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
The Overexpression of Subtilisin Enzyme Using Mutations on Transition State Regulatory Proteins of AprE Promoter and Development of Bacillus subtilis Host System
AprE Promoter전이상태 조절인자 변이주를 이용한 공업적 효소의 과발현과 고초균 숙주계의 개발
Abstract
Bacillus subtillis strains with transition state regulator mutations and a spore mutation were developed for the overexpression of apsE and for the enhancement of expression level. Among the many regulator genes, degU and hpr were chosen as a representative positive and negative regulator for the aprE, respectively. Spo II G was used for the construction of asporogeneous strains. All the mutants were constructed from two protease-deleted strain DB104 and the apsE gene was transformed with an integration vector pMK101. DB104(deg
Bacillus subtilis를 재조합이종 단백질 생산에 응 용하고 그 발현효율의 향상을 위하여, aprE 프로모 터를 모델계로서 이에 작용하는 전이상태 조절유전 자 변이와 포자형성변이의 영향을 살펴보았다. 사용 된 균주는 두 개의 프로테아제가 제거된 DB104이며, 조절인자 변이주는
Keywords