Effect of Incorrectly Estimated Parameters on the Control of Specific Growth Rate in E. coli Fed-Batch Fermentation

An Exponetial feeding strategy has been frequently used in fed-batch fermentation of recombinant E. coli. In this feeding scheme, growth yield and initial cell concentration, which can be erroneously determined, are needed to calculate the feed rate for controlling specific growth rate at the set point. The effect of the incorrect growth yield and initial cell concentration on the control of the specific growth rate was theoretically analyzed. Insignificance of the correctness of those parameters for the control of the specific growth rate was shown theoretically and experimentally.