Cloning of a Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) from a Scylliorhinus torazame

두툽상어에서 tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) 유전자의 클로닝

  • 김차순 (부산대학교 분자생물학과) ;
  • 배수경 (부산대학교 분자생물학과) ;
  • 김규원 (부산대학교 분자생물학과) ;
  • 김영진 (부산대학교 분자생물학과)
  • Published : 1996.12.01

Abstract

Angiogenesis is a fundamental process by which new blood vessels are formed. It is essential in embryo development, and wound healing. Furthermore, malignant tumor growth and metastasis are also angiogenesis-dependent. In the catilage tissue, normal angiogenesis process is suppressed. In fact, it was reported that angiogenesis-inhibitory substances were isolated from the extracts of cow and shark catilage tissue. In order to isolate genes involved in the regulation of angiogenesis from a catilage fish, we constructed a shark cDNA library from Scylliohinus torazame. We then screened the library using hyman tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) gene as a probe. Among the 4 X 10$^{4}$ plaques screened, we isolated 2 positive clones (T-1, T-2). Restriction enzyme analysis revealed that the T-1 clone contains 0.8 kb cDNA insert, and the T-2 clone contains 1.2 kb and 2.2 kb inserts, respectively. Further DNA sequence analysis shows that the DNA sequence of the T-1 clone is 53% homologous to that of the human TIMP-1 gene.

Keywords

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