이담자균 효모의 성분화과정중 인지질의 작용과 배지조성의 제한이 성분화에 미치는 영향

The Action of Phospholipids and Effect of Medium Composition During Sexual Differentiation Process in Heterobasidiomycetous Yeast Rhodospotidium toruloides.

  • 정영기 (동의대학교 미생물학과) ;
  • 강원대 (동의대학교 미생물학과) ;
  • 남수완 (동의대학교 미생물학과)
  • 발행 : 1996.09.01

초록

The action of phospholipid on the rhodotorucine A(RH.A) acceptance by heterbasidiomyceteous yeast Rhodosporidium toruloides mating type a cells and the effect of medium composition during sexual differentiation were investigated. Activation of trigger peptidase(TPase)was very sensitive to the originated phospholipid from R. toruloides and was more sensitive to phospholipid liposome made up of phospholipi. Phospholopod present on the membrance of mating type a cells consists of phospatidylglycerol(PG), phosphatidylethanolamine(PE), phospatidylcholine(PC), phospatidylinositol(PI), and phosphatidylserine(PS) of 12.9, suprisingly 45.4, 11.0, and 13.9%, respectively. As the result of using C-1 and N-1 mediums which limited C and N sources capable of inhibiting the synthesis of phospholipid, it resulted inhibiting sexual dlfferentiation and production of Rh.A from mating type Acells.

키워드

참고문헌

  1. J. Gen. Appl. Microbiol. v.13 Studies on sexuality of Rhodotorula Bano, I.
  2. J. Gen. Appl. Microbiol. v.24 Morphological change in the early stage of the mating of Rhodosporidium toruloides M1057, a strain of mating type a Abe, K.;I. Kusaka.;S.Fukui.
  3. Agric. Biol. Chem. v.43 Structure of rhodotorucine A, a peptidyl factor inducing mating tube formation in Rhodosporidium toruloides Kamiya. Y.;A. Sakurai. S.;S. Tamura.;A. Abe.;S. Fukui.
  4. Kor. J. Appl. Microbiol. Biotechol. v.23 Characterization of sexual agglutination and involvement of cel-surface protein sexual cell-cell interaction of heterobasidiomycetous yeast Jeong. Y. K.;T. H. Lee;Y. L. Choi;W. D. Kang
  5. J. Bacteriol. v.151 Role of metabolism of the mating pheromone in sexual differentiation of the heterobasidiomycetous Rhodosporidium toruloides Miyakawa, T.;M. Nishihara;E. Tsuchiya;S. Fukui
  6. J. Bacteriol. v.169 Purification and characterization of a Ca^{2+}-dependent membrane peptidase involvede in the signaling of mating pheromone in Rhodosporidium toruloides Miyakawa, T.;Y. K. Jeong;M. Kaji;E. Tsuchiya;S. Fukui
  7. Kor. J. Appl. Microbiol. Biotechnol. v.20 The comparision of the characteristics of partially purified internal invertase by mating type in the Heterobasidiomycetous yeast Jeong, Y. K.;B. W. Kim
  8. J. Kourean Soc. Food Nutr. v.21 Purification of internal invertase in Rhodosporidium toruloies Mating type A cells Jeong, Y. K.;B. H. Ryu
  9. Eur. J. Biochem. v.169 Interaction with phospholipids of a membrane thiol peptidase that is essential for the signal transduction of mating pheromone in Rhodosporidium toruloides Jeong, Y. K.;T. Miuakawa;A. Imabayashi;E. Tsuchiya;S. Fukui
  10. Kor. J. Appl. Microbiol. Biotechnol. v.22 Relation of Ca{2+}-ATPase and Trigger peptidase(TPase) that are membrane protein in a differentiation process on heterobasidiomycetous yeast Jeong, Y. K.;T. H. Lee;K. T. Jeong.