Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Studies on the Adenosine Deaminase Inhibitor Producing Actinomycetes J144-K

방선균 J-144K가 생산하는 Adenosine Deaminase Inhibitor에 관한 연구

  • Jun, Hong-Ki (Department of Microbiology, Pusan National University) ;
  • Kim, Sam-Woong (Department of Microbiology, Pusan National University) ;
  • Jo, Young-Bae (Department of Microbiology, Pusan National University) ;
  • Yeeh, Yeehn (Institute of Basic Sciences, Inje University)
  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 김상웅 (부산대학교 자연과학대학 미생물학과) ;
  • 조영배 (부산대학교 자연과학대학 미생물학과) ;
  • 이인 (인제대학교 기초과학연구소)
  • Published : 1996.06.01
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Abstract

In the screening of actinomycetes culture filtrate for inhibitor of adenosine deaminase, a novel inhibitor was found in a cultured broth of strain J-144K. The optimum conditions for the adenosine deaminase inhibitor production from the isolated strain J-144K were evaluated. This strain showed the maximum yield of adenosine deaminase inhibitor when grown at pH 7.0 and 30$\circ$C for 60 hours in the medium of 1.0% dextrose, 0.5% yeast extract, 0.5% peptone and 0.1% KH$_{2}$PO$_{4}$ under the aerobic condition. Through the activated charcoal extraction, methanol fractionation, Dowex 50 H$^{+}$ X-8 ion exchange column chromatography, Dowex CI$^{-}$ X-8 ion exchange column chromatography, and Sephadex G-15 gel filtration procedures, this inhibitor was purified with three materials.

Keywords

References

  1. Lancet v.2 Adenosine deninase deficiency in two patients with severely impaired cellular immumity Giblett, E. R.;Anderson, J. E.;Cohen, F.;Pollara, B.;Meuwissen, H. J.
  2. Biochemistry v.26 Immunoaffinity purification and fluorescence studies of human adenosine deaminase Philips, A. V.;Robbins, D. J.;Coleman, M. S.;Barkley, M. D.
  3. Biochem, J. v.195 Purification and radioimmunoassay of adenosine deaminase from human leukaemic granulocytes Wigiaton, D. A.;Coleman, M. S.;Hutton, J.J.
  4. J. Biol. Chem. v.251 Human adenosine deaminase, Distribution and properties Van der Weyden, M. B.;Kelley, W. N.
  5. Biochemistry v.22 Adenosine Deaminase : Solvent Isotope and pH Effects on the Binding of Transition-State and Ground-State Analogue Inhibitors Kurz, L. C.;Frieden, C.
  6. Biochemistry v.25 Transition-State Stabilization by Adenosine Deaminase : Structural Studies of Its Inhibitory Complex with Deoxycoformycin Frick, L. F.;Smal, E.;Baker, D. C.;Wolfenden, R.
  7. Biochemistry v.24 Spontaneous Epimerization of (S)-Deoxycoformycin, and Interaction of (R)-Deoxy-Coformycin, (S)-Deoxycoformycin, and 8-Keto-deoxycoformycin with Adenosine Deaminase, Schramm, V. L.;Baker, D. C.
  8. Enzyme Inhibitors of Mocrobial Origin Umezawa, H.
  9. Biochemistry v.16 Influence of Substituent Ribose on Transition State Affinity in Reactions Catalyxed by Adenosine Deaminase Wolfenden, R. Wentworth, D. F.;Mitchell, G. N.
  10. J. American Chemical Society v.96 Synthesis of Coformycin Ohno, M.;Yagisawa, N.;Kondo, S.;Maeda, K.;Umezawa, H.
  11. J. Antibiotics v.20 Mode of Inhibition of Coformycin on Adenosine Deaminase Sawa, T.;Fukagawa, Y.;Homma, I.;Takeuchi, T.;Umezawa, H.
  12. J. Antibiotics v.20 Studies on Formycin and Formycin B in Cells of Ehrlich Carcinoma; E. coli Umezawa, H.;Sawa, T.;Fukagawa, Y.;Homma, I.;Ishizuka, M.;Takeuchi, T.
  13. J. Science v.35 Adenosine deaminase ingibitor of microbial origin,Partially purification of adenosine deaminase inhibitor from Streptomyces sp Jun, H. K.
  14. J. Biol. Chem. v.223 The Ingibition of Adenosine Deaminase by 8-Azaguanine in Vitro Feigelson, P.;Davidson, J. D.
  15. Biochim. Biophys. Acta v.178 Competitive Inhibition of Adenosine Deaminase by Urea and Amodine Derivatives Ronca, G.;Ronca-Testoni, S.
  16. Biochim, Biophys. Acta v.132 Competitive Ingibition of Adenosine Deaminase by Urea, Guanidine, Biuret and Guanylurea Ronca, G.
  17. Biochemistry v.11 no.15 Ingibition of Adenosine Deaminase by Alcohols Derived from Adenine Nucleosides Lerner, L. M.;Ross, R. R.
  18. J. Biol. Chem. v.167 Dofferemtial spectrophotometry of purine compounds by means of specific enzymes, Ⅱ. Determination of adenine compounds Kalckar, H. M.