Tea fungus 발효음료 제조시 발효계의 미생물상

Microflora Occurring in the Fermentation by Tea Fungus

  • 최미애 (대구효성카톨릭 대학교 식품영양?과) ;
  • 최경호 (대구효성카톨릭 대학교 식품영양?과) ;
  • 김정옥 (부산여자대학교 화학과)
  • 발행 : 1996.03.01

초록

10%의 sucrose를 첨가한 홍차추출물에 tea fungus를 접종하여 (30^{\circ.)$에서 정치배양하여 발효하였다. 14일 동안의 발효에 의해 배양액의 전표면에 7~8mm의 두꺼운 피막이 생겼으며, 배양액의 pH가 2.5 부근으로 저하되었다. 발효 과정 중 아래쪽의 배양액에서는 효모(Saccharomyces cerevisiae and Eeniella sp.)와 여러종류의 세균(Bacillus subtilis, Kurthia zopfli, Gluconobacter oxydans와 Deinococcus sp.)이 분리되었다. 반면에 피막 부위에서는 Acetobacter aceti의 단일균주가 분리되었으며 이 세균은 통상의 Acetobacter와는 달리 점질상의 덩어리로 성장하였다. 발효음료는 달고 새콤한 맛과 약간의 달콤한 과일향을 나타내었다. 이상의 결과로부터 tea fungus에 의한 홍차발효는 다양한 미생물이 공동으로 작용하여 진행되는 symbiotic acetate발효로서, 발효음료는 생물학적으로 안전할 뿐만아니라 적당한 발효조건에 의해 좋은 향미를 갖춘 유망한 음료로 판단되었다.

Black tea extractbsupplemented with 10% sucrose was fermented by fungus at 30$\circ$C. A pellicle thick as 7$\sim$8 mm covered entire surface of the medium and the wxtract converted to acidic beverage(abbreviated below as fermented black tea) by 14 days of fermentation. It was a kind of acetic acid fermentation depending on symbiotic microorganisms. During the fermentation strains of yeasts(Saccharomyces cerevisiae and Eeniella sp.)and bacteria(Bacillus subtilis, Kurthia zopfii, Gluconobacter oxydans and Deinicoccus sp.) were isolated from aqueous layer. Contrastly to it, a bacterial strain(Acetobacter aceti) was isolated from thick pellicle. The bacteria grew as a viscouse cluster on solid agar medium differently from usual strains of A. aceti. Fermented black tea had sweet-sour taste and sweet smell.

키워드

참고문헌

  1. Nutrition Reviews v.41 no.10 The future of Fermented Foods Hesseltine, C.W.
  2. Ecol. of Food and Nutr. v.24 A review of traditional fermented foods, condiments and beverages in nigeria Uzogara, S.G.;Agu, L.N.;Uzogara, E.O.
  3. Frod. Research International. v.27 no.3 Nutritional significances of fermented food Steinkaraus, K. H.
  4. J. Food Technology Production of pito, a Nigerian fermented beverage Ekunadayo, J.A.
  5. J. Jpn. Soc. Nutr. Food. Sci. v.47 Lactic acid bacteria and yeast from Kefir Yoshida, T.;Toyoshima, K.
  6. Microbiology of fermented foods v.1 Brian J. B. Wood
  7. Prog. Ind. Microbiol English cyder making Beech. F. W.
  8. J. of chromatography v.555 High-performance liquid chromatography of the phenolic compounds in the juice of some french cider apple varieties Delage, E.;Bohuon, G.;Bason, A.;Drilleau, J. F.
  9. Dairy Ind. v.36 Enumeration and viablity of L. bulgaricus and S. thermophilus in yoguris Davis, J.G.;Ashton, I.R.;Mccaskill, M.
  10. MS thesis, Graduate school, Gyeongsang National University Potent anticarcinogenic effect of the extracts from Artemisia capillaris and Poeona suffruticosa Bahn, K. N.
  11. Bergey's manual of determinative bacteriology John G.H.;Wlliams;Wilkins
  12. 微生物의 分利法 山里一英;宇田川俊一;兒玉徹;林地敏樹
  13. The yeasts Kreger-van R.
  14. Antonie van Leeuwenhoek v.64 Genetic organization of Acetobacter for acetic acid fermention Beppu T.
  15. Zeitschrift fur lebensmittel-unter suchung und Forschung v.198 no.3 Influence of different sugars on the metabolism of the tea fungus Reiss, J.
  16. Verbrau-cherdienst v.33 no.10 The tea fungus fermented drink Benk. E.