Journal of Microbiology

  • 제34권4호
  • /
  • Pages.349-354
  • /
  • 1996
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Cloning and Expression in E. coli of the HOPDA Hydrolase Gene from Pseudomonas sp. P20

  • 발행 : 1996.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Pseudomonas sp. P20 is a natural isolate which is capable of degrading biphenyl and 4-chlorobiphenyl. From a clone of pCK1022 harboring pcbCD genes of Pseudomonas sp P20, a pcbD gene encoding 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA) hydrolase was subcloned in Escherichia coli XL-1-Blue by using pBluescript SK(+) vector. The 2.8-kb HindII fragment harboring the pcbD gene cloned in pCK 1024 had a single site for each of XhoI, SalI, BstXI, and XbaI restriction enzymes. Escherichia coli CK1024 had a single site for each of XhoI, SalI, BstXI, and XbaI restriction enzymes. Escherichia coli CK1024 carrying pCK0124 degraded HOPDA to benzoate and 2-hydroxypenta-2, 4-dienoate by HOPDA hydrolase encoded by pcbD gene as effectively as E coli CK 1022 HARBORING pcbCD genes.

키워드

참고문헌

  1. Agr. Biol. Chem. v.42 Alcaligenes and Acinetobacter strains capable of degrading polychlorinated biphenyls Furukawa, K.;F. Matsumura;K. Tonomura
  2. J. Bacteriol. v.171 Molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: Some soil bacteria possess a highly conserved bph operon Furukawa, K.;N. Hayase;K. Taira;N. Tomizuka
  3. J. Bacteriol. v.166 Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes Furukawa, K.;T. Miyazaki
  4. J. Bacteriol. v.172 Pseudomonas putida KF715 bphABCD operon encoding biphenyl and polychlorinated biphenyl degradation: cloning, analysis, and expression in soil bacteria Hayase, N.;K. Taira;K. Furukawa
  5. Gene v.130 Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation Hofer, B.;L. D. Eltis;D. N. Dowling;K. N. Timmis
  6. Appl. Environ. Microbiol. v.56 Identification and localization of 3-phenylcatechol dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase genes of Pseudomonas putida and expression in Escherichia coli Khan, A. A.;S. K. Walia
  7. Appl. Environ. Microbiol. v.57 Expression, localization, and functional analysis of polychlorinated biphenyl degradation genes cbpABCD of Pseudomonas putida Khan, A. A.;S. K. Walia
  8. Kor. J. Microbiol. v.32 Cloning and expression of pcbCD genes in Escherichia coli from Pseudomonas sp. DJ-12 Kim, C.-K.;T.-K. Sung;J.-H. Nam;Y.-C. Kim;J.-K. Lee
  9. Biophy. Biochem. Res. Comm. v.226 Characterization of the pcbC gene encoding 2,3-dihydroxybiphenyl dioxygenase from Pseudomonas sp. P20 Kim, C.-K.;E. Kim;J.-C. Chae;Y. Kim
  10. Appl. Environ. Microbiol. v.62 Genetic structure of the genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading Pseudomonas sp. strain DJ-12 Kim, E.;Y. Kim;C.-K. Kim
  11. J. Bacteriol. v.171 Cloning and sequencing of two tandem genes involved in degradation of 2,3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyldegrading soil bacterium Pseudomonas sp. strain KKS102 Kimbara, K.;T. Hashimoto;M. Fukuda;T. Koana;M. Takagi;M. Oishi;K. Yano
  12. J. Bacteriol. v.171 Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation Mondello, F. J.
  13. Kor. J. Appl. Microbiol. Biotechnol. v.22 Cloning of pcb genes in Pseudomonas sp. P20 specifying degradation of 4-chlorobiphenyl Nam, J.-H.;C.-K. Kim
  14. J. Microbiol. Biotechnol. v.5 Cloning and expression of pcbC and pcbD genes responsible for 2,3-dihydroxybiphenyl degradation from Pseudomonas sp. P20 Nam, J.-H.;H.-M. Oh;C.-K. Kim
  15. Agr. Biol. Chem. v.50 Purification and some properties of a 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolyzing enzyme from Pseudomonas cruciviae S93 B1 involved in the degradation of biphenyl Omori, T.;K. Sugimura;H. Ishigooka;Y. Minoda
  16. Kor. J. Appl. Micobiol. Biotechnol. v.24 Purification and characterization of 2,3-dihydroxybiphenyl dioxygenase from recombinant E. coli CK1092 Park, H.-N.;Y. Kim;Y.-C. Kim;C.-K. Kim;J.-Y. Lim
  17. Molecular cloning. A laboratory manual(2nd ed.) Sambrook, J.;E. F. Fritsch;T. Maniatis
  18. J. Bacteriol. v.175 Identification of a catabolic transposon, Tn4371, carrying biphenyl and 4-chlorobiphenyl degradation genes in Alcaligenes eutrophus A5 Springael, D.;S. Kreps;M. Mergeay
  19. J. Biol. Chem. v.267 Analysis of bph operon from the polychlorinated biphenyl-degrading strain of Pseudomonas pseudoalcaligenes KF707 Taira, K.;J. Hirose;S. Hayashida;K. Furukawa
  20. Kor. J. Microbiol. v.30 Divergence of the cbp genes in 4-chlorobiphenyl catabolizing bacteria Youn, D.-J.;J.-J. Han;C.-K. Kim;Y. Kim