Characterization of Azomonas agilis PY101, a Cadmium-Resistant Strain Isolated from Anyang Stream

  • You, Kyung-Man (Department of Biology, Korea University) ;
  • Lee, Ji-Hyun (Department of Biology, Korea University) ;
  • Kim, Jeong-Kook (Department of Biology, Korea University) ;
  • Hah, Nam-Ju (Department of Pharmacy, Sahmyook University) ;
  • Lee, Yung-Nok (Department of Biology, Korea University) ;
  • Park, Yong-Keun (Department of Biology, Korea University, Graduate School of Biotechnology, Korea University)
  • Published : 1996.09.01

Abstract

A cadimium-resistant strain isolated from Anyang stream, Azomonas agilis PY101 exhibited strong resistance to 1000 ppm of cadmium ion $(Cd^{2+})$. A agilis PY101 also exhibited resistance to various antibiotics, such as amoxicillin, amplicillin, bacitracin, cefazolin, erythromycin, penicillin, tetracycline, and vancomycin. In the presence of $Cd^{2+}$, the growth of A. agilis PY101 started after an extended lag phase and produced a green-fluorescent pigment induced by cadmium. The dramatic decrease (approximately 400ppm) of concentration of $cd^{2+}$ in the culture medium during the growth phase of A. agilis PY101 was confirmed by the inductively coupled plasma-atomic emission spectrophotometer. Transmission electron microscopic analysis revealed that A. agilis PY 101 actively accumulated $Cd^{2+}$ in the cytoplasm.

Keywords

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