Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Lipopolysaccharide Yields from Rhodobacter capasulatus with indirect ELISA

  • Published : 1996.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to $NH_ 4CI$ concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM $CaCI_ 2$, the LPS yield was 16.5 $\mu$g/mg DW, five times the yield without calcium.

Keywords

References

  1. J. Immunol. Meth. v.82 An enzyme linked immunosorbent assay(ELISA) for the measurement of antibodies to different parts of the gram negative lipopolysaccharide core region Appelmelk, B.J.;Db.M. Verweij-Van Vught. MacLaren;L.G. Thijs
  2. Inject. Immun. v.52 protection by serum antibodies in exprimental non-typable Haemophilus influenzae otitis media Barenkamp. S.J.
  3. Appl. Environ. Microbiol. v.59 Effects of nutrients on exopolysaccharide production and surface properties of Aeromonas salmoni- cida Bonet, R.;M.D. Simon-Pujol;F.Congregado
  4. Plant Physiol. v.84 The isolation and partial characterization of the lipopolysaccharides from several Rhizobium trifolii mutant affected in root hair infection Carlson, R.W.;Jauh-Lin Duh;E. Turnbull;B. Hanley;B.G. Rolfe;M.A. Djorjevic
  5. Plant Physiol. v.83 Detection of glycosylated and deglycosylated extensin precursor by indirect competitive ELISA Conrad, T.A.;D.T.A. Lamport;R. Hammerschmidt
  6. Hyg Abl. Orig. Suppl. v.1 Die Isolierung schwefelfreier Purpurbakterien. Zentralb Bakteriol. Parasitenk. Infektionskr Drews, G.
  7. Advances in Microbial Physiology Effects of environment on bacterial wall content and composition Ellwood, D.C.;D.W. Tempest;Rose, A.H.(Ed.);Tempest, D.W.(Ed.)
  8. Arch. J. Microbiol. v.139 The capsule and slime olysaccharides fo the wild type and a phage resistant mutant of Rhodo- pseudomonas capsulata St. Louis Flammann, H.T.;J.R. Golecki;J. Weckesser
  9. J. Bacteriol. v.159 Characterization of the cell wall and outer membrane of Rhodopseudomonas capsulata Flamann, H.T.;J. Wechesser
  10. Infect. Immun. v.16 Biological activities of lipopolysaccharides and lipid A from Rhodo- spirillaceae Galanos, C.;J. Roppel;J. Weckesser;T.E. Tietschel;H. Mayer
  11. Endotoxin research series v.2 Lipid A precusor Ia(compound 406) and Rhodobacter capsulatus lipopolysaccharide:potent endotoxin antagonists in the human system in vitro Harald, L.
  12. J. Bacteriol. v.129 H₂metabolism in the photo-synthetic bacte-rium Rhodopseudomonas capsulata : H₂production by growing cultures Hillmer, P.;H. Gest
  13. Intan. J. Syst. Bacte-riol. v.38 Electrophoretic analysis of lipopoly-saccharides of purple non-sulfur bacteria Krauss, J.H.;J. Weckesser;H. Mayer
  14. J. Gen. Microbiol. v.133 Factors that control the rate of exopolysaccharide production by Agrobacterium radiobacter NCIB 1b1883 Linton, J.D.;D.S. Jones;S. Woodard
  15. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O.H.;N.J. Rosebrough;A.L. Farr;R.J. Randall
  16. Adv. Microb. Physiol. v.20 The outer membrane of gramnegative bacteria Nikaido, H.;T. Nakae
  17. Microbiol. Rev. v.49 Molecular basis of bacterial outer membrane permeability Nikaido, H.;M. Vaara
  18. Arch. Microbiol. v.134 Detec-tion of capsule and slime polysaccharide layes in two strains of Rhodopseu-domonas capsulata Omar, A.S.;H.T. Flamma;J.R. Gorecki;J. Weckesser
  19. Arch. Microbiol. v.134 Lipo-polysaccharides of two strains of phototrophic bacterium Rhodopseudomonas capaulata Omar, A.S.;H.T. Flamman;D. Borowiak;J. Weckesser
  20. Acta. Pathol. Micro-biol. Scand. v.26 Antigen-antibody reaction in gels Ouchterlony, A.
  21. Microbiology v.140 Identifica-tion of chromosomal genes located downstream of dclD that affect the requirement for calcium and lipopolysaccharide layer of Rhizobium leguminosarum Poole, P.S.;N.A. Schofield;C.J. Reid;E.M. Drew;D.L. Walshaw
  22. Appl. Environ. Microb. v.59 Effects of nutrents on exopolysaccharide production and surface properties of Aeromonas salmoni-cida Ramon, B.;M.D. Simon-Pujol;F. Congregado
  23. J. Clin. Microbiol. v.33 Comparision of lipopolysaccharide and outer membrane protein-lipopolysaccharide extracts in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis infection Riezu-Boj, J.I.;I. Moriyonn;J.M. Blasco;C.M. Marin;R. Diaz
  24. Ann. Rev. Microbiol. v.33 lipopolysaccharides of photosynthetic prokaryotes Salton, E.
  25. Infect. Immun. v.21 Antibody response to antigens distinet from smooth lipopolysaccharide complex in Brucella infection Schurig, G.G.;L.M. Jones;S.L. Speeth;D.T. Berman
  26. Appl. Environ. Microbiol. v.58 Anaerobic production of extra-cellular polysaccharide by bulyrivibrio fibrisolvens nyx Wachenheim, D.E.;J.A. Patterson
  27. J. Bacteriol. v.130 Lipophilie -anti-gen in Rhodospirillum tenue Weckesser, J.;G. Drews;R. Indira;H. Mayer
  28. Ann. Rev. Microbiol. v.33 Lipopolysaccharides of photosynthetic prokaryotes Weckesser, J.;g. Drews
  29. Intern. J. Syst. Bacteriol. v.33 Lipopolysaccharide of Rhodocyelus purpureus : Taxanomic implication Weckesser, J.;H. Mayer;E. Metz;H. Biebel
  30. Mikrobiologie v.10 Lipopolysaccharide aus phototrophen bakte- rien. Forum Naturwiss Weckesser, J.;H. Mayer
  31. FEMS Microb. Rev. v.54 Different lipid A types in lipolysaccharides of phototrophic and related non-photrophic bacteria Weckesser,J.;H. Mayer
  32. J.bacteriology v.109 Chemical analysis of and degradation studies on the call wall lipopolysaccharide of Rhodobacter capsulata Weckesser, J.;g. Drews;I. Frome
  33. Z. Naturforsch v.7b Uber die Extraktion von Bakterien mit Phenol/Wasser Westphal, O.;O. Luderitz;F. Bister
  34. Methods in carbohydrate chemistry v.5 Bacterial lipopolysaccharide. Extraction with phenol-water and further application o this procedure Westphal, O.;K. Jann;Whistler, R.I.(Ed.);Miller, J.N.(Ed.);Wolform, M.I.(Ed.)
  35. Anal. Biochem. v.160 A method for purification of bac-terial R-type lipopolysasccharides (lipooligosaccharide) Wu, L.;C.M. Tsai;C.C. Frasch