Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Detection of L-Xylosone and its Physiological Effects in Saccharomyces cerevisiae

  • Seok, Yeong-Jae (Laboratory of Biophysics, Department of Microbiology, College of Natural Science and Research Center for Molecular Microbilogy, Seoul National University) ;
  • Yang, Kap-Seok (Laboratory of Biophysics, Department of Microbiology, College of Natural Science and Research Center for Molecular Microbilogy, Seoul National University) ;
  • Kang, Ju-Gyeong (Laboratory of Biophysics, Department of Microbiology, College of Natural Science and Research Center for Molecular Microbilogy, Seoul National University) ;
  • Kim, Seong-Tae (Laboratory of Biophysics, Department of Microbiology, College of Natural Science and Research Center for Molecular Microbilogy, Seoul National University) ;
  • Huh, Won-Ki (Laboratory of Biophysics, Department of Microbiology, College of Natural Science and Research Center for Molecular Microbilogy, Seoul National University) ;
  • Kang, Sa-Ouk (Laboratory of Biophysics, Department of Microbiology, College of Natural Science and Research Center for Molecular Microbilogy, Seoul National University)
  • Published : 1996.06.01
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Abstract

L-Xylosone was detected as its quinoxaline derivative in the degradation solution of dehydro-L-ascorbic acid. The production rate of L-xylosone was much faster in aerated phosphate-cirate buffer (pH 5. 6) than in pure water. L-Xylosone and dehydro-L-ascorbic acid were identified in the crude extracts of Saccharomyces cerevisiae. The concentration of L-xylosone in the crude cell extracts was calculated to be about 0.2 nmol $(mg protein)^{-1}$. When L-xylosone was added to asynchronous culture of S. cerevisiae, it inhibited primarily the synthesis of protein and RNA. Examination of the effect of L- xylosone on synchronous culture of the yeast indicated that L-xylosone inhibited the initiation of DNA replication and that the cells were arrested at $G_1$, stage of cell division cycle. These results suggested a possibility that L-xylosone can act as an inhibitor of cell growth.

Keywords

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