Journal of Microbiology

  • 제34권2호
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  • Pages.158-165
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  • 1996
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Genetic Relationship between the SPT3 Gene and RAS/cAMP Pathway in Yeast Cell Cycle Control

Genetic relationship between the SPT3 gene and ARS/cAMP pathway in yeast cell cycle control

  • 발행 : 1996.06.01
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초록

The signal transduction pathways through the RAS gene product and adenyl cyclease play a critical role in regulation of the cell cycle in yeast, Saccharomyces cerevisiae. We examined the genetic relationship between the spt3 gene and ras/cAMP pathway. A mutation in the SPT3 gene suppressed cell cycle arrest at the G1 phase caused by either an inactivation of the RAS or CYR1 gene which encodes a yeast homologue of human ras proto-oncogene or adenyl cyclase, respectively. The phenotypes such as sporulation and heat shock resistancy, that resulted from a partial inactivation of the RAS or CYR1 genes, were also suppressed by the spt3 mutation. Expression of the SSA1 gene encoding one of th heat shock proteins (Hsp70) can be induced by heat shock or nitrogen starvation. Expression of this gene is derepressed in cry1-2 and spt3 mutants. The bcy 1 mutation repressed by the bcy1 mutation, but not in spt3 mutants. These results suggest that the SPT gene is involved in expression of genes that are affected by the RAS/cAMP pathway.

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참고문헌

  1. Cell v.16 Evidence for transposition of dispersed repetitive DNA families in yeast Cameron, R.R.;E.Y. Loh;R.W. Davis
  2. Genes & Devel. v.6 SPT3 interact with THIID to allow normal transcription in Saccharomyces cerevisiae Eisenman, D.M.;K.M. Arndt;S.L. Ricupero;J.W. Rooney;F. Winston
  3. Mol. Cell Biol. v.4 Differential regulation of the 70K heat shock gene and related genes in Saccharomyces cerevisiae Ellwood, M.S.;E.A. Craig
  4. Genetics v.78 Genes controlling meiosis and spore formation in yeast Esposito, M.;R.E. Esposito
  5. Proc. Natl. Acad. Sci. v.78 Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerewisiae Guarente, L.;M. Ptashne
  6. Science v.183 Genetic control of cell division cycle in yesat; a model Hartwell, L.H.;J. Cloutti;J.R. Pringle;B.J. Reid
  7. Mol. Cell. Biol. v.8 Specific early G1 block accompained with stringent response in Saccharomyces cerevisiae Iida, H.;I. Yahara
  8. Mol. Cell. Biol. v.2 Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila Ingolia, T.D;M.R. Slater;E.A. Craig
  9. J. Bacteriol. v.153 Transformation of intact yeast cells treated with alkali cations Ito, H.;Y. Fukuda;K. Murata;A. Kimura
  10. Cell v.43 DNA sequence and characterization of the Saccharomyes cerevisiae gene encoding adenylate cyclase Kadaoka, T.;D. Broke;M. Wigler
  11. Cell v.37 Genetic analysis of yeast RAS1 and RAS2 genes Kadaoka, T.;S. Power;C. McGill;O. Fasano;J. Strathern;J. Broach;M. Wigler
  12. Genetics v.82 Regulation of mating and meiosis in yesat by mating type locus Kassir, T.;G. Simchen
  13. Genetics v.76 Mutation affecting sexual conjugation and related processes in Saccharomyces cerevisiae MacKay, V.I.;T.R. Manney
  14. Molecular Cloning; A Laboratory Manuual Manniatis, T.;E.F. Fritsch;J. Sambrook
  15. Exp. Cell. Res. v.146 Control of cell division in Saccharomyces cerevisiae mutants defective in adentlate cyclase and cAMP-dependent protein kinase Matsumoto, K.;I. Uno;T. Ishikawa
  16. Proc. Natl. Acad. Sci. v.79 Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase Matsumoto, K.;I. Uno;Y. Oshima;T. Ishikawa
  17. Cell v.31 Initiation of meiosis in yeast mutants defective in adenylate cyclase and protein kinase Matsumoto, K.;I. Uno;t. Ishikawa
  18. Experiments in Molecular Genetics Miller, J.H
  19. Genetics v.123 Isolation and characterization of temperature-sensitive mutations in the RAS2 and CYR1 genes in Saccharomyces cerevisiae Mistuzawa, H.;I. Uno;T. Oshima;T. Ishikawa
  20. Mol. Cell. Biol. v.9 Positive and negative regulation of basal expression of a yeast HSP70 gene Park, H.O.;E.A. Craig
  21. Molecular Biology of Yesat; life cycle and inheritance Pringle, J.R.;L.H. Hartwell;Strathern J.N.(ed.);E.W. Jones(ed.);J.R. Broach(ed.)
  22. Cell v.50 Three different genes in Saccharomyces cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase Toda, T.;S. Cameron;P. Sass;M. Zoller;M. Wigler
  23. Mol. Gen. Genet. v.191 The isolation and genetic analysis of sporulation deficient mutants in Saccharomyces cerevisiae Tsuboi, M
  24. Yeast Genetics Laboratory course mannul for methods in yesat genetics Sherman, F.;G.R. Fink;J.B. Hicks
  25. Curr. Genet. v.12 Control of the G1/Go transition and Go protein synthesis in Saccharomyces cerevisiae Shin, D.Y.;I. Uno;T. Ishikawa
  26. Mol. Cell. Biol. v.7 Heat shock response of Saccharomyces cerevisiae altered in cyclic AMP-dependent protein phosphorylation Shin, D.Y.;K. Matsumoto;H. Iida;I. Uno;T. Ishikawa
  27. J. Biol. Chem. v.258 Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast Uno, I.;K. Matsumoto;K. Adachi;T. Ishijkawa
  28. J. Biol. chem. v.258 Characterization of a cyclic nucleotide phosphodiestease-deficient mutant in yeast Uno, I.;K. Maatsumoto;T. Ishikawa
  29. Genetics v.107 Mutation affecting Ty-mediated expression of the HIS4 gene of Saccharamyces cerevisiae Winston, F.;D.T. Chaleff;B. Valent;G.R. Fink
  30. Cell v.39 The SPT3 gene is equired for normal tanscription of Ty element in Saccharomyces cerevisiae Winston, F.;K.J. Durbin;G.R. Fink
  31. Nucleic Acids Res. v.14 Analysis of the yeast SPT3 gene and identification of its products, a positive regulator of Ty transcription Winston, F.;P.L. Minehart