Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Molecular Cloning and Expression of Shiga-Like ToxinIIGene (slt-II) from an Isolate of Healthy Korean Native Bovine Feces, Escherichia coli KSC109

  • Published : 1996.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

By PCR amplification using the sequence of the previously cloned shiga-like toxin II DNA, a gene encoding it has been cloned from an isolate of healthy Korean native bovine feces, Escherichia coli KSC109. The nucleotide sequence s included tow open reading frames coding for 319 and 89 amino acids corresponding to A and B subunits, respectively. Comparison of the nucleotide and predicted amino acid sequences of newly cloned gene (slt-II) with those of others in the SLT-II family revealed completely identical homology with SLT-II cloned previously from bacteriophabe DNA of E. coli 933 derived from a patient with hemorrhagic colities. In addition, the sequence homology of SLT-II with SLT-II variant form bovine was more than 95% at both the nucleotide and protein levels. Overexpression of SLT-II recombinant gene by induction with IPTG using an E, coli hostvector, system was conducted and the correctly processed products with active mature form exhibited 1000-fold higher cytotoxycity for Vero cells than that form original strain.

Keywords

References

  1. J. Gen. Microbiol. v.136 Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxinⅡ family Gannon, V.P.J.;C. Teerling;S.A. Masri;c.L. Gyles
  2. J. Clin. Microbiol. v.12 Quantitative microtiter cytotoxicity assay for Shigella toxin Gentry, M.K.;J. M. Dalrymple
  3. Microb. Pathog. v.5 Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O19 : H21 isolated from a patient with the hemolytic uremic syndrome Ito, H.;A. Terai;H. Kurazono;Y. Takeda;M. Nishibuchi
  4. FEMS Microbiol. Lett. v.44 Nucleotide sequence analysis and comparison of the structural genes for Shiga-lkie toxin Ⅰ and Shiga-like toxin Ⅱ encoded by bacteriophages from Escherichia coli 933 Jackson, M.P.;R.J. Neill;A.D. O'Brien;R.K. Holmes;J.W. Newland
  5. Clin. Microbiol. Rev. v.2 Infection by verotoxin-producing Escherichia coli Karmali, M.A
  6. Microbiol. Immunol. v.37 Cloning and sequencing of two new verotoxin 2 variant genes of Escherichia coli isolated from cases of human and bovine diarrhea Lin, Z.;S. Yamasaki;H. Kurazono;M. Ohmura;T. Karasawa;T. Inoue;S. Sakamoto;T. suganami;T. Takeoka;Y. Taniguchi;T. Takeda
  7. J. Biol. Chem. v.262 Identifiation of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type Ⅰ Lindberg, A.A.;J.E. Brown;N. Stromberg;M. Westling-Ryd;J.E. Schultz;K.A. Karlsson
  8. J. Biol. Chem. v.262 Glycolipid binding of purified and recombinant Escherichia coli produced Verotoxin in vitro Lingwood, C.A.;H. Law;S. Richardson;M. Petric;J.L. Brunton;S. De Grandis;M. Karamali
  9. Molecular cloning: a laboratory manual Maniatis,T.;E.F. Fitsch;J. Sambrook
  10. FEMS Microbiol. Lett. v.44 Escherichia coli strains isolated from pigs with edema disease produce a variant Shiga-like toxin Ⅱ Marques, L.R.M.;J.S.M. Peiris;J.S. Cryz;A.D. O'Brien
  11. Annu. Rev. Biochem. v.54 Mechanism and control of transcription initiation in prokaryoktes McClure, W.R
  12. Microbiol. Reve. v.51 Shiga and shigalike toxins O'Brien, A.D.;R.K. Holmes
  13. Science v.226 Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea O'Brien, A.D.;J.W. Newland;S.F. Miller;R.K. Holmes;H.W. Smith;S.B. formal
  14. Annu. Rev. Mcirobiol. v.39 Protein secretion in Escherichia coli Oliver, D
  15. Infect. Immun. v.63 Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric shiga-like toxin type Ⅱ operons Paton, A.W.;A.J. Bourne;P.A. Manning;J.C. Paton
  16. J. Clin. Microbiol. v.34 Enterobacter cloacae producing a shiga-like toxin Ⅱ-related cytotoxin associated with a case of hemolytic-uremic syndrome Paton, A.W.;J.C. Paton
  17. Microb. Pathog. v.13 Cloning and nucleotide sequence of a variant Shiga-like toxin Ⅱ gene from Escherichia coli OX3 : H21 isolated from a case of Sudden Infant Death Syndrome Paton, A.W.;J.C. Paton;M.W. Heuzenroeder;P.N. Goldwater;P.A. Manning
  18. Microb. Pathog. v.15 Polymerase chain amplification, cloning and sequencing of variant Escherichia coli Paton, A.W.;J.C. Paton;P.A. Manning
  19. Proc. Natl. Acad. Sci. v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;S. Nicklen;A.R. Coulson
  20. Infect. Immun. v.59 Two copies of shiga-like toxinⅡ-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157 : H-strain E 32511 Schmidt, C.K.;M.L. McKee;A.D. O'brein
  21. Infect. Immum. v.61 Shiga-like toxin Ⅱ-related cytoxins in Citrobacter freundii stains form humans and beef samples Schmidt, H.;M. Montag;J. Bockemuhl;J. Heemsemann;H. Karch
  22. Infect. Immun. v.53 Two-toxin-coverting phages from Escherichia coli O127 : H7 strain 933 encode antigenically distinct toxins with similar biological activites Strockbine, N.A.;L.R.M. Margues;J.W. Newland;H. W. Smith;R. K. Holmes;A.D. O'Brien
  23. J. Bacteriol. v.170 Cloning and sequencing of a shiga-like toxin type Ⅱ variant from an Escherichia coli strain responsible for edema disease of swine Holmes, A.D. O'Brien