Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

Solid-Phase Extraction of Sulfamerazine from Shrimp Residue and Determination by Reversed Phase High Performance Liquid Chromatography

  • Jang, Won-Cheoul (Department of Chemistry, College of Natural Science, Dankook University) ;
  • Heo, Gang-Joon (Laboratory of Aquatic Animal Disease, College of Veterinary Medicine, Chungbuk National University)
  • Published : 1996.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The focus of this study was to investigate the suitable analytical methods for measurement of sulfamerazine and its metabolite in shrimp hepatopancreas and tail tissue, in addition to the methods for the optimization of solid-phase extraction cartridge conditions and the elucidation of sulfamerazine concentrations in aqueous buffer using HPLC with UV and EC detectors. Compared with UV detector the EC detector appears to be 10 times more sensitive than that of the UV detector. After the shrimp was exposed to 10 ppm sulfamerazine, the accumulation levels of sulfamerazine and its metabolite in tail tissue, which is edible portion, were considerably lower than 0.1 ppm. The data indicate that sulfamerazine continues to be a candidate for use at levels of sulfamerazine concentration used in aquaculture of shrimp.

Keywords

References

  1. J. Chromatogr. v.414 Rapid method for the determination of elther plasma sulphapyridine or sulfamethoxazole and their acetyl metabolites using high performance liquid chromatography Astbury,C.;Dixon,J.S.
  2. Xenobiotica v.18 Pharmacokinetics of sulphadimethoxine in the lobster, Homarus americanus, following intrapericardial administration Barron,M.G.;Gedutis,C.;James,M.O.
  3. Ver. Hum. Toxicol. v.29 An outline of penaeid shrimp culture methods including infectious disease problems and priority during treatments Bell,T.A.;Lightner,C.V.
  4. J. Wildl. Dis. v.19 Chitinoclastic bacteria associated with shell diseae in penaeus shrimp and blue crab(Callinectes sapicus) Cook,D.W.;Lofton,S.R.
  5. Aquaculture v.16 Accumulation and deplition of oxytetracycline in juvenile shite shrimp(Penaeus setiferus) Corliss,J.P.
  6. Fish. Bull. v.76 Diseases, parasites and toxic responses of commercial penaeid shrimps of the Gulf of Mexico and South Atlantic Coast of North America Cough, J.A.
  7. J. Chromatogr. v.196 Determination of sulfanilic acid in the presence of sulfanilamide and some sulfa drugs by reversedphase ion-pair high performance liquid chromatography Doerr, R.C.;Parris,N.;Parks,O.W.
  8. Ver. Hum. Toxicol. v.30 Disposition of sulfacimethoxine in lobster(Horarus Amerioaus) James, M.O.;Barron, M.G.
  9. Biochem. J. v.136 The metabolism of four sulfonamides in cows Nielson,P.
  10. Drug Develop. and Indust. Pharm. v.12 Quantitation of sulfacetamide, sulfadiazino, sulfamerazine, and sulfamethazine in various combinations using high performance liquid chromatography Parasrampurta,J.;Gupta, V.D.
  11. Prog. Fish. Cult. v.48 Status of ishery chemicals in 1985 Schnick, R.A.;Meyer, F.P.;walsh, D.F.
  12. Drug Metabol. and Dis. v.7 Metabolism and disposition by the rat of S-sulfadiazine alone and in thd presence of trimethoprin Woolley,J.L.;Siegel, C.W.