Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
권호 표지

Non-Adrenergic Non-Cholinergic Responses of Gu mea- Pig Tracheal Smooth Muscle

기니피그 기도 평활근의 비아드레날린성 비꼴린성 반응에 관한 연구

  • 조은용 (조선대학교 의과대학 흉부외과학교실) ;
  • 최형호 (조선대학교 의과대학 흉부외과학교실, 조선대학교 의과대학 생리학교실) ;
  • 전제열
  • Published : 1996.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

The neurogenic responses of tracheal smooth muscles to electrical field stimulation (EFS) is biphasic, consisting firstly of cholinergic contraction followed by a slow and sustained relaxation. It is well known that a sustained relaxation involves the inhibitory non-adrenergic non-cholinergic systems. This study was done to Investigate the relaxing agents and their action mechanisms by use of an organ bath with plati- ilum . The tracheal smooth muscle relaxation due to EFS was suppressed by L-NAME, the WO (Nitric Oxide) synthase inhibitor, and these effects were reversed by L-arginine, the precursor of NO. Also, L-WAME (HG-nitro-L-arginine methyl ester) increased the basal tension. Nitroprusside, the NO-donor, suppressed the tracheal basal tension greatly. Methylene blue, the inhibitor of guanylate cyclase, decreased EFS-induced relaxations and increa ed basal tension. Forskolin and isoprenaline, which are activators of adenylate cyclase, suppressed tracheal basal tension in the same way as nitroprusside. TEA (tetraethylammonium), the non-specific K'channel blocker, and apamin, the Ca"-activated K'channel blocker, increased tracheal basal tension and EFS-induced relaxations. Our results indicate that Pr3 Is released upon stimulation of the NANC (Won Adrenergic Won Cholinergic) nerves in guinea-pig tracheal smooth muscle and that the release of NO related with the K+ channel, as well as the release of other inhibitory agents< e. g.)VIP (Vasoactive Intestinal Polypeptide), PHI (Peptide Histidine Isoleusine) > mediated via CAMP (cyclic Adenosine Monophosphate) may be Involved In sustained relaxation.

기도평활근을 전장 자극하면 콜린성 수축에 이은 느리고도 지속적 인 이완성 반응이 유발된다. 지속적 인 이완성 반응은 억제성인 비아드레날린성 비콜린성 신경 섬유에 의해서 야기되는 것으로 알려져있다. 그러나 이러한 신경 말단에서 분비되는 신경 전달물질에 대해서는 아직도 실험한 동물 및 사용 조직에 따라 다르게 보고되고 있다. 본 실험은 기 니피그 기도 평 활근에서 전장 자극을 주어 이완성 반응에 관여 하는 인자 및 그 작용 기전을 알아보고자 하였다. 전장 자극으로 유발된 이완성 반응은 L-NAME에 의 해서 억제되 었으며 L-arginine에 의 해서 부분적으로 회복되 었다. 또한 L-WAME은 기초장력을 증가시켰 다. Hitroprusside는 윤상근의 기초 장력을 완전히 억제하였으며, methylene blue는 전장 자극으로 유발 된 이완성 반응을 억제함과 동시에 기초장력을 증가시켰다 Forskolin과 isoprenaline는 nitroprusside와 똑같이 기도 평활근의 기초 장력을 크게 억제하였다. TEA와 apamin은 기도 평활근 기초 장력 및 전장 자극으로 유발된 이완성 반응을 모두증가시켰다. 이상의 실험 결과로 보아 기니피그 기도 평활근 비교 감성 비콜린성 신경 섬\ulcorner 말단에서는 K' 통로와 관련하여 WO가 분비되며 이외에도 CAMP를 매개로 하는다른억제성 신경 전달물질(ex. WP,만Tl)이 분비되리라사료된다.

Keywords

References

  1. Airway smooth muscle in health and disease(1st ed.) Structure of airway smooth muscle and its innervation Gabella,G.;Coburn,R.F.
  2. Lung v.159 Nonadrenergic inhibitory innervation of the lung Richardson,J.B.
  3. Am Rev Resp Dis v.134 State of art. Neural control of human airways in health and disease Barnes,P.J.
  4. Br J Pharmacol v.79 Evidence against vasoactive intestinal polypeptide(VIP) as a dilator and in favour of substance P as a constrictor in airway neurogenic responses Karlsson,J.A.
  5. Arch Int Pharmacodyn v.303 Nonadrenergic noncholinergic control of mucus secretion in airways Webber,S.E.
  6. Trends Pharmacol Sci v.11 Modulation of neurogenic inflammation: novel approches to inflammatory diseases Barnes,P.J.;Belvis,M.G.;Rogers,D.F.
  7. J Pharmacol and Experimental Ther v.256 Release of nitric oxide upon stimulation of nonadrenergic noncholinergic nerves in the rat gastric fundus Boeckxstaens,G.E.;Pelckmans,P.A.;Bogers,J.J.(et al.)
  8. J Physiol v.461 Nitric oxide involvement in the peptide VIP-associated inhibitory junction potential in the guinea-pig ileum He,X.D.;Goyal,R.K.
  9. Br J Pharmacol v.112 Noradrenergic-nitrergic interactions in the rat anococcygeus muscle : evidence for postjunctional modulation by nitric oxide Kasakov,L.;Belai,A.;Vlaskovska,M.;Brunstock,G.
  10. Life Science v.48 $N^G$-nitro-L-Arginine inhibits non-adrenergic non-cholinergic relaxation in rabbit urethral smooth muscle Dokita,S.;Morgan,W.R.;Wheeler,M.A.;Yoshida,M.;Latifpour,J.;Weiss,R.M.
  11. Arch Int Pharmacodyn Ther v.280 Non-adrenergic non-cholinergic nervous control of gastrointestinal motility patterns Abrahamson,H.
  12. J Appl Physiol v.61 VIP and PHI and their role in non-adrenergic inhibitory responses in isolated human airways Palmer,J.B.;Cuss,F.M.C.;Barnes,P.J.
  13. Br J Pharmacol v.96 The effects of vasoactive intestinal peptide(VIP) antagonists, and VIP and peptide histidine isoleucine antisera on non-adrenergic, non-cholinergic relaxtions of tracheal smooth muscle Ellis,J.L.;Farmer,S.G.
  14. Am J Physiol v.262 Nitric oxide mediates the neural nonadrenergic, noncholinergic relaxation of pig tracheal smooth muscle Kannan,M.S.;Johnson,D.E.
  15. J Appl Physiol v.56 Cholinergic and non-adrenergic mechanisms in human and guinea-pig airways Taylor,S.M.;Pare,P.D.;Schellenburg,R.R.
  16. Br J Pharmacol v.40 Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic nervous in the gut Burnstock,G.;Campbell,G.;Satchell,D.;Rand,M.J.
  17. Br J Pharmacol v.52 A non-adrenergic inhibitory nervous pathway in guinea-pig trachea Coleman,R.A.;Leby,G.P.
  18. Br J Pharmacol v.83 Adenosine deaminase antagonizes inhibitory responses to adenosine and non-adrenergic, non-cholinergic inhibitory nerve stimulation in isolated preparations of guinea-pig trachea Satchell,D.G.
  19. Am J Physiol v.262 Nitric oxide as a mediator of nonadrenergic noncholinergic neurotransmission Sanders,K.M.;Ward,S.M.
  20. J Physiol v.444 Effect of nitric oxide on circular muscle of the canine small intestine Stark,M.E.;Bauer,A.J.;Szurszewski,J.H.
  21. Lancet v.343 Nitric oxide:mediator, murdurer, and medicine Anggard,E.
  22. J Physiol v.445 Spontaneous release of nitric oxide inhibits electrical, Ca²+ and mechanical transients in canine gastric smooth muscle Ozaki,H.;Blondfield,D.P.;Hori,M.;Publicover,N.G.;Kwto,I.;Sanders,K.M.
  23. Pharmacol Rev v.43 Nitric oxid: physiology, pathophysiology, and pharmacology Moncade,S.;Palmer,R.M.J.;Higgs,E.A.
  24. Nature v.368 Nitric oxide directly activates calcium-dependent potassium channels in vascular smooth muscle Bolotina,V.M.;Najibl,S.;Palacino,J.J.;Pagano,P.J.;Cohen,R.A.
  25. Bioche Biophy Res Com v.163 Direct regulation of smooth muscle contractile elements by second messengers Nishimura,J.;Van Breemen,C.
  26. Eur J Pharmacol v.109 Effect of vasoactive intestinal polypeptide(VIP) on cyclic AMP level and relaxation in rat isolated aorta Schoeffter,P.;Stoclet,J.C.
  27. J Pharmacol Exp Ther v.253 Vasoactive intestinal peptide(VIP) as transmitter of inhibitory neurons of the gut : evidence from the use of selective antagonists and VIP antiserum Grider,J.R.;Rivier,J.J.
  28. Gastroenterology v.104 Involvement of nitric oxide in the inhibitory innervation of the human isolated colon Boeckxstanes,G.E.;Pelckmans,P.A.;Herman,A.G.;Van Maercke,Y.M.