Abstract
In an attempt to evaluate the effect of St. Thomas' hospital cardioplegic solution (STH) I and II on the cultured rat myocardial cells, beating rate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Irtrazolium MTT) and lactate dehydrogenase activity were investigated, and also light and electron microscopic studies were carried out. After rat myocardial cells were cultured for 72 hours, cells were treated with STH I or with STH H solution for 30 and 120 min. and thereafter myocardial cells were cultured in control medium for 24 hours. The results obtained were as follows : 1. Beating rate was 154 times per min. in control group, 141 times per min. in STH I solution(for 2. 120 min.)-treated group and 145 times in STH ll solution (for 120 min.)-treated group. MTT absorbances by MTT assay were 102% in STH I solution (for 120 min.)-treated group and 93% in STH ll solution (for 120 min.)-treated group compared with control group. 3. The amount of lactate dehydrogenase released into the medium were 123% in STH I solution (for 120 min.)-treated group and 109% in STH H solution (for 120 min.)-treated group compared with control group. 4. In the light microscopy examination, myocardial cells showed no differences between experimental and control groups in their number and shape. 5. In the electron microscopy examination, myocardial cells treated with STH I solution showed fewer destroyed mitochondria compared to STH ll solution-treated group. These results suggest that both 51. Thomas'cardioplegic solution STH 1 and STH H have no cytotoxicity on cultured rat myocardial cells, but STH H solution has more protective effect on myocardial cells compared to STH I solution.
St. Thomas' 심정지액 I과 II가 백서 배양심근세포에 미치는 영향을 알아보기 위하여 심장 박동수, MTT 정량, LDH 정량 및 형태학적 관찰을 시행하였다. 백서의 심근 세포를72시간 배양한 후St. Thomas' 심정지액 I과 H에 30분및 120분간 처리한후24 시간 동안 배양하여 다음과 같은 결과를 얻었다. 1. 심근 세포 박동수는 대조군이 분당 154회, St. Thomas' 심정지액 I 에 120분 처리한 실험군에서는 147회, II 120분처리군에서는 145회로대조군에 비하여 유의한 차이를 보이지 않았다. 2. MTT 정량 결과St. Thomas' 심정지액 1120분 처리군에서는대조군의 102%로나타났으며. R B2O 분처리군에서는 93%로 대조군에 비하여 유의한 차이를 보이지 않았다. 3. LDH정량 결과 St. Thomas' 심정지액 1120분처리군에서는대조군의 123%로나타났으며, R 120 분처리군에서는 109%로 약간의 증가를 보였다. 4. 광학현미경적 관찰결과세포수및 세포 형태에 있어서 대조군과 실험군간에 차이를 보이지 않았다. 5.전자현미경적 관찰 결과St. Thomas'심정지액 I 처리군에서는소수의 미토콘드리아파괴가관찰되 었으며, II 처리군에서는대조군과유사한소견을보였다. 이상의 실 瘟嘯倖?종합하여 볼때, St. Thomas'심정지액 I과 II는백서 배양심근세포에 세포독성 을 나타내지 않았으나,전자현미경적 관찰결과심정지액 II가심정지액 I에 비하여 심근보호효과가 더 양호한 것으로 생각된다.
