Effects of Glucose, SOD and Catalase Levels During the In Vitro Culture in Medium on In Vitro Developmental Rates of Porcine Oocytes

Glucose, SOD, Catalase 첨가가 돼지 수정란의 체외발생에 미치는 영향에 관한 연구

The study was conducted to determine the optimal glucose, superoxide dimutase(SOD) and catalase levels during the in vitro culture of porcine oocytes matured and fertilized in vitro for morulae and blastocyst development. Oocytes were cultured for 0~8 days in TCM-199 medium supplemented with 20% FCS, different glucose, SOD and catalase levels. The results are summairzed as follows ; 1. The in vitro developmental rates of porcine oocytes cultured in TCM-199 medium containing 0.1, 0.3, 0.5, 1.0, 3.0 mM glucose levels 0~3 and 0~8 days after insemination were 22.8, 24.2, 21.9, 20.0, 12.1 and 21.9, 26.7, 25.0, 22.6, 16.7%, respectively. 2. The in vitro developmental rates of porcine oocytes cultured in TCM-199 medium containing 100, 200, 300, 500 $\mu\textrm{g}$/ml SOD levels 0~3 and 0~8 days after insemination were 16.7~23.3 and 16.7~25.0%, respectively. High levels of SOD(500 $\mu\textrm{g}$/ml) significantly reduced the rates of molurae and blastocysts stage(P<0.05). 3. The in vitro developmental rates porcine oocytes cultured in TCM-199 medium containing 100, 200, 300, 500 $\mu\textrm{g}$/ml catalase levels 0~3 and 0~8 days after insemination were 18.8~26.7 and 19.4~28.1%, respectively, and there was significant differences on the development to the molurae and blastocysts stage among the cumulus cells and glucose levels.