한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제20권3호
- /
- Pages.259-269
- /
- 1996
- /
- 1226-5284(pISSN)
간세포 배양을 이용한 뱀장어 Vitellogenin 합성에 대한 웅성호르몬의 영향
Induction of Vitellogenin Synthesis by Androgens in Cultured Hepatocytes of the Eel, Anguilla japonica
초록
To establish whether or not androgens is responsible for the induction of vitellogenin(Vg) synthesis and secretion, primary hepatocytes prepared from immature eels were used. The results are follows: 1. Eel hepatocytes were prepared using a collagenase perfusion technique. The isolated cells attached efficiently to fibronectin-coated dishes and subsequently formed monolayers in serum-free medium. These cultures maintained in medium for 10 days with minimal cell loss. 2. Estradiol-17