한국가금학회지 (Korean Journal of Poultry Science)

  • 제23권4호
  • /
  • Pages.177-183
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  • 1996
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

DNA Marker를 이용한 한국 재래닭의 유전특성 분석

Analysis of Genetic Characteristics of Korean Native Chicken Using DNA Marker

  • 이학교 (서울대학교 동물자원과학과) ;
  • 이성진 (서울대학교 동물자원과학과) ;
  • 황규춘 (서울대학교 동물자원과학과) ;
  • 정일정 (서울대학교 동물자원과학과) ;
  • 박용호 (서울대학교 동물자원과학과) ;
  • 손시환 (서울대학교 동물자원과학과) ;
  • 신영수 (서울대학교 동물자원과학과) ;
  • 오봉국 (서울대학교 동물자원과학과) ;
  • 한재용 (서울대학교 동물자원과학과)
  • 발행 : 1996.12.01
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초록

This study was conducted to analyze genetic characteristics of Korean Native Chicken three lines classified on the basis of the feather color and appearance (Red, Yellow, and Black) using DNA fingerprinting method. To estimate the genetic relatedness among breeds and similarities within breeds, we collected blood samples from Korean Native Chicken (KNC), Rhode Island Red (RIR), White Leghorn (WL), and Cornish(CN) and obtained genomic DNA from the blood of 10 individuals randomly selected within the breeds and lines. The genomic DNA samples were digested with restriction enzymes (Hinf J, Hae Ill) and hybridized with various probes (Jeffreys' probes 33.15, 33.6 and M13) after Southern transfer. Genetic similarities within breeds were characterized by band sharing (BS) value, estimated by the DFP band pattern between the pair of lanes. BS values within WL, RIR, and KNC were 0.82, 0.70 and 0.56, respectively. Relative genetic diversity (BS value) of KNC was higher than those two breeds (WL, RIR). Estimation of genetic similarity between KNC lines and control breed (RIR) was 0.32, whereas similarity within KNC lines (6 groups) was 0.50. In this analysis, KNC was showed to have a highly genetic diver-sity at the DNA level, and to be closer in genetic distance to RIR (0.67) than any other breeds.

키워드

참고문헌

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