한국가금학회지 (Korean Journal of Poultry Science)

  • 제23권2호
  • /
  • Pages.61-69
  • /
  • 1996
  • /
  • 1225-6625(pISSN)
  • /
  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

Lac Z 유전자가 전이된 원시생식세포 주입에 의한 병아리 생식기내 유전자 발현

Expression of Lac Z Gene in Young Chick Gonad by the Transtected Primordial Germ Cell Injection

  • 한재용 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 서동삼 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 홍영호 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 정동기 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 최강덕 (서울대학교 농업생명과학대학 동물자원과학과) ;
  • 신영수 (신구전문대 축산과)
  • 발행 : 1996.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Primordial germ cells (PGCs) were manipulated as part of the system to produce transgenic chickens. PGCs were isolated from the germinal crescent of developmental stage 6 to 8 donor emhryos of the Korean Native Ogol Chickens (KNOC). These PGCs were transfected with plasmid DNA containing the lac Z gene by liposome mediated transfection methods. The lac Z gene was transfected and expressed in the PGCs. These transfected PGCs were injected into the germinal crescent of White Leghorn embryos (stage 6 to 8). The injected transfected PGCs migrated via the circulatory system into the future gonad and expression observed in the gonads of 3 day old chick. Of the 47 embryos and 3 day old chickens, one positive PGCs gonad from sacrificed young chickens was detected by appearance of blue cells. Plasmid DNA with the foreign gene was incorporated into the population of germ cells in the gonad. These results demonstrate that PGCs can he transfected and then transferred for colonization into the gonad, and show the potential to ultimately manipulate the genetic material of the chicken gernline.

키워드

참고문헌

  1. Sci v.243 Germline transmission of exogenous genes in chicken Bosselman,R.A.;Hsu,R.Y.;Boggs,T.;Hu,S.;Bruszewski,J.;Ou,S.;Kozar,L.;Martin,F.;Green,C.;Jacobson,F.;Nicolson,M.;Schultz,J.A.;Semon,K.M.;Rishell,W.;Stewart,R.G.
  2. Devel v.117 Germline chimeric Chickens from dispersed donor blastodermal cells and compromised recipient embryos Carsience,R.S.;Clark,M.E.;Gibins,A.M.V.;Etches,R.J.
  3. Mol Cell Biol v.7 High-efficiency transformation of mammalian cells by plasmid DNA Cfen,C.;Okayama,H.
  4. Therio v.41 Evidence for pluripotency of bovine primordial germ cell-derived cell lines maintained in long-term culture Cherny,R.A.;Merei,J.
  5. J Embryol Exper Morph v.65 Avian primordial germ cells are of epiblastic origin Eyal-Giladi,H.;Ginsburg,M.;Farbarov,A.
  6. Bethesda Research Laboratory Focus v.11 Cationic liposome-mediated transfection Felgner,P.L.;Holm,M.
  7. Anat Rec v.185 The origin, migration and morphology of the primordial germ cells in the chick embryo Fujimoto,T.;Ukeshima,A.;Kiyofuji,B.
  8. Devel v.101 Primordial germ cells of the young chick blastoderm originate from central zone of the area pellucida irrespective of the embryo-forming process Ginsburg,M.;Eyal-Giliadi,H.
  9. J Morph v.88 A series of normal stages in the development of the chick embryo Hamburger,V.;Hamilton,H.L.
  10. Asian-Austr J Anim Sci v.6 Stable transformation of cultured chicken cells Han,J.Y.;Shin,Y.S.;Shoffner,R.N.;Guise,K.S.
  11. Asian-Austr J Anim Sci v.7 Gene transfer by manipulation of primordial germ cells (PGCs) in the chicken Han,J.Y.;Shoffner,R.N.;Guise,K.S.
  12. Int J Dev Biol v.35 Embryonic expression of β-actin-lacZ hybrid gene injected into the fertilized ovum of the domestic fowl Naito,M.;Agata,K.;Otsuka,K.;Kino,K.;Ohta,M.;Hirose,K.;Perry,M.M.;Eguchi,G.
  13. J Repro Fert v.102 Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring Naito,M.;Tajima,A.;Tagami,T.;Yasuda,Y.;Kuwana,T.
  14. Mol Repro Devel v.39 Production of germline chimeric chickens, with high transmission rate of donor-derived gametes, produced by transfer of primordial germ cells Naito,M.;Tajima,A.;Yasuda,Y.;Kuwana,T.
  15. Anat Rec v.222 Extragonadal distribution of primordial germ cells in the early chick embryo Nakamura,M.;Kuwana,T.;Miyayama,Y.;Yoshinaga,K.;Fujimoto,T.
  16. Devel v.108 Production of somatic and Germline Chimeras in the chicken by transfer of early blastodermal cells Petitte,J.N.;Clark,M.E.;Liu,G.;Verrinder-Gibbins,A.M.;Etches,R.J.
  17. Roux's Archi Devel Biol v.179 Capacities reproductrices et descendance de poulets ayant subi untransfert de poulets ayant subi untransfert de cellules germinales primordiales durant la vie embryonnaire Reynaud,G.
  18. Virology v.157 Transgenic chickens: insertion of retroviral genes into the chicken germline Salter,D.W.;Smith,E.J.;Hughes,S.H.;Wright,S.E.;Crittenden,L.B.
  19. M.S. Thesis Primordial germ cell transfer in the chicken Gallucs domesticus Shuman,R.M.
  20. Poultry Sci v.65 Gene transfer by avian retroviruses Shuman,R.M.;Shoffner,R.N.
  21. Proc of the 4th World Cong on Genetics Applied to Livestock Prod v.ⅩⅥ Infection of primordial germ cells with defective retrovirus and their transfer to the developing embryo Simkiss,K.;Vick,L.;Luke,G.;Page,N.;Savva,D.
  22. Therio v.41 Production of chimaeric bovine embryos Stokes,T.M.;Cherny,R.A.;Brandon,M.R.
  23. Therio v.40 Production of germ line chimera by transfer of primordial germ cells in the domestic chicken (Gallus domesticus) Tajima,A.;Naito,M.;Yasuda,Y.;Kuwana,T.
  24. Poultry Sci v.73 Somatic and germline chicken chimeras obtained from brown and white leghorns by transfer of early blastodermal cells Thoraval,P.;Lasserre,F.;Coudert,F.;Dambrine,G.
  25. Poultry Sci Abst Long-term culture of chick primordial germ cells isolated form the blastodisc and germinal crescent Tsai,H.;Wentworth,B.C.
  26. Devel v.103 Analysis of germ line development in the chick embryo using an antimouse EC cell antibody Urven,L.E.;Erickson,C.A.;Abbott,U.K.;McCarrey,R.J.
  27. Proc of the Royal Soc of Lond (B) v.251 Transgenic birds from transformed primordial germ cells Vick,L.;Ying,L.;Simkiss,K.
  28. Poultry Sci v.68 Manipulation of avian primordial germ cells and gonadal differentiation Wentworth,B.C.;Tsai,H.;Hallett,J.H.;Gonzales,D.S.;Rajcic Spasojevic,G.
  29. J Reprod Fert v.96 A method to obtain avian germ-line chimaeras using isolated primordial germ cells Yasuda,Y.;Tajima,A.;Fujimoto,T.;Kuwana,T.