Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Determination of Niflumic Acid in Human Urine by Gas Chromatography/Negative Chemical lonization Mass Spectrometry

  • Myung, Seung-Woon (Doping Control Center, Korea Institute of Science and Technology) ;
  • Kim, Myung-Soo (Doping Control Center, Korea Institute of Science and Technology) ;
  • Cho, Hyun-Woo (Department of Chemeistry, KyungGi University) ;
  • Park, Jong-Sei (Doping Control Center, Korea Institute of Science and Technology)
  • Published : 1996.12.01
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Abstract

A sensitivity method has been developed for the detection and determination of niflmic acid(NA) in human urine. Samples were extracted with diethylether. Flunixin (FN) was added to the sample prior to extraction as an internal standard. Niflumic acid was converted to its methyl derivative and analyzed by capillary gas chromatography/negative chemical isonization mass spectrometry. Using selected ion monitoring (SIM), the levels of NA down to 5 pg/ml could be detected in 5 ml spiked urine sample. Calibration curve was linear over the range of 0.5 ppm-50 ppm. The recovery of niflumic acid from urine at 40 pg/ml was to be $91.7{\pm}3.8(n=3)$ and the coefficient of variation was 4.1%.

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References

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