A New Streptothricin Family Antibiotic Producing Streptomyces Spp. Snus 8810-111 ; Characterization of The Producing Organisms, Fermentation, Isolation, and Structure Elucidation of Antibioitics

  • Goo, Yang-Mo (Department of Pharmacy, Seoul National University) ;
  • Kim, Ok-Yun (Department of Pharmacy, Seoul National University) ;
  • Joe, Young-Ae (Department of Pharmacy, Seoul National University) ;
  • Lee, Young-Bok (Department of Pharmacy, Seoul National University) ;
  • Ju, Jeongho (Department of Pharmacy, Seoul National University) ;
  • Kim, Beom-Beom-Tae (Department of Chemistry, Seoul National University) ;
  • Lee, Youn-Young (Department of Chemistry, Seoul National University)
  • Published : 1996.04.01

Abstract

A new streptothricin family antibiotic producing Streptomyces spp. SNUS 8810-111 was isolated from a soil sample. Study of its morphological and physiological characters indicated that the antibiotic producing organism was a Streptomyces spp. Taxonomical studies suggested that the organism might belong to the genus streptomyces gougeroti. The organism produced antibiotics most in calcium carbonate-tryptic soy broth. The active principles were recovered from the broth with a cation exchange resin and eluted from the resin with HCI. Cellulose column chromatography gave two active principles.$^1H-^1H$ Homo-COSY study on the first compound revealed four structural components. Total hydrolysis of the antibiotic with HCI allowed isolation of $\beta-lysine$. From these data the antibiotic was found to be streptothricin D. The other compound showed one additional signal in the .$^1H$NMR and the $^{13}C$ NMR spectra. The signal was from a methyl group attached to a nitrogen atom. Comparison of the NMR signals with those of streptothricin D suggested that the compound was N-methyl-streptothricin D which was a new compound in the family of streptothricin antibiotics.

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