BMB Reports
- Volume 29 Issue 2
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- Pages.163-168
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- 1996
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- 1976-670X(eISSN)
Purification of the Glycosylated Polyphenol Oxidase from Potato Tuber
- Kwon, Do-Yoon (Department of Biotechnology, Chung-Ang University) ;
- Kim, Woo-Yeon (Department of Biotechnology, Chung-Ang University)
- Published : 1996.03.31
Abstract
Glycosylated polyphenol oxidase was purified from potato tuber using ammonium sulfate fractionation, Sephadex G-100, and concanavalin A Sepharose column chromatography. Two or three types of polyphenol oxidase were separated on concanavalin A Sepharose. Type I and II polyphenol oxidases did not bind to concanavalin A Sepharose. Type I seemed to be an aggregated form of polyphenol oxidase. Type III polyphenol oxidase, which is presumed to be glycosylated because it was bound to concanavalin A Sepharose and eluted with