BMB Reports
- Volume 29 Issue 2
- /
- Pages.111-115
- /
- 1996
- /
- 1976-670X(eISSN)
Characterization of a Novel Glutathione S-Transferase from Pseudomonas sp. DJ77
- Jung, U-Hee (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
- Cho, Young-Sik (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
- Seong, Hark-Mo (Department of Microbiology, College of Natural Science, Chungbuk National University) ;
- Kim, Seong-Jae (Department of Microbiology, College of Natural Science, Chungbuk National University) ;
- Kim, Young-Chang (Department of Microbiology, College of Natural Science, Chungbuk National University) ;
- Chung, An-Sik (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
- Published : 1996.03.31
Abstract
A novel glutathione S-transferase from Pseudomonas sp. DJ77 was expressed in E. coli and purified by glutathione-affinity chromatography. The enzyme was composed of two identical subunits. The molecular size of the enzyme was 42 kDa by sephadex G-150 gel permeation chromatography and Mr of each subunit was 23 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis. pI value of the enzyme was approximately 5.8 by isoelectric focusing. This enzyme showed the highest activity toward 1-chloro-2,4-dinitrobenzene as the electrophilic substrate. The relative activities toward p-nitrobenzyl chloride and 1,2-dichloro-4-nitrobenzene were 3.8% and 1.3% of the activity toward 1-chloro-2,4-dinitrobenzene, respectively.