Korean Journal of Clinical Pharmacy (한국임상약학회지)

Korean College Of Clinical Pharmacy (한국임상약학회)
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The Effect of Cephalosporins on the Stability of Gentamicin and Tobramycin in Human Serum

세파로스포린계 약물이 겐타마이신, 토브라마이신의 혈청중 안정성에 미치는 영향

  • Kim, In Wha (College of Pharmacy, Sookmyung Women's University) ;
  • Lee, Suk Hyang (College of Pharmacy, Sookmyung Women's University) ;
  • Shin, Hyun Taek (College of Pharmacy, Sookmyung Women's University) ;
  • Kim, Myung Min (Department of Clinical Pharmacology Research Center Samsung Medical Center and Samsung Biomedical Research Institute) ;
  • Choi, Kyung Eob (Department of Clinical Pharmacology Research Center Samsung Medical Center and Samsung Biomedical Research Institute)
  • 김인화 (숙명여자대학교 약학대학) ;
  • 이숙향 (숙명여자대학교 약학대학) ;
  • 신현택 (숙명여자대학교 약학대학) ;
  • 김명민 (삼성생명과학연구소 임상약리센터) ;
  • 최경업 (삼성생명과학연구소 임상약리센터)
  • Published : 1996.12.01
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Abstract

The in vitro inactivation of gentamicin and tobramycin by four cephalosporins (cefotetan, cefuroxime, cefodizime, cefotiam) in human serum was investigated. Each cephalosporin was added to human serum samples containing gentamicin sulfate or tobramycin sulfate. Blank samples containing only aminoglycosides were used as controls. Samples were stored at -20, 4 and $25^{\circ}C$ and were analyzed for aminoglycoside concentrations by fluorescence polarization immunoassay ($TDxFLx^{TM}$ system) at 0, 2, 4, 8, 12, 24, 48 and 72 hours after mixing. The serum containing cefotiam stored at $25^{\circ}C$ showed significant inactivation of gentamicin by $12\%$ at 72 hours. The results indicate that cefotitan, cefuroxime and cefodizime do not inactivate gentamicin and tobramycin while cefotiam inactivates gentamicin.

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