Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Characterization and DNA Structure Analysis of Replication Origin of Misgurnus mizolepis

미꾸라지의 복제원점에 대한 특성 및 구조 분석

  • Lim Hak-Seob (Department of Biotechnology and Bioengineering, National Fisheries University of Pusan) ;
  • Kim Moo-Sang (Department of Biotechnology and Bioengineering, National Fisheries University of Pusan) ;
  • Seok Young-Seon (Department of Biotechnology and Bioengineering, National Fisheries University of Pusan) ;
  • Park Sang-Dai (Department of Molecular Biology, Seoul National University) ;
  • Lee Hyung-Ho (Department of Biotechnology and Bioengineering, National Fisheries University of Pusan)
  • 임학섭 (부산수산대학교 생물공학과) ;
  • 김무상 (부산수산대학교 생물공학과) ;
  • 석영선 (부산수산대학교 생물공학과) ;
  • 박상대 (서울대학교 분자생물학과) ;
  • 이형호 (부산수산대학교 생물공학과)
  • Published : 1996.02.01
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Abstract

Previously, as an effort to make an autonomously replicating expression vector in fish, an ARS (autonomously replicating sequence) was cloned from MAR (matrix attachment region) of Misgurnus mizolepis. The DNA fragment composed of 443 base pairs contains ARS core consensus sequences, topoisomerase II consensus sequences, and A or T box sequences which are homologous to the known consensus sequences originated from other organisms. The clond ARS, as other DNA replication origins, contains inverted repeat sequences and several potential hairpin loop structures. These consensus sequences and hairpin structures may serve as recognition signals for regulatory proteins of DNA replication initiation.

물고기에서 효과적인 발현 vector의 구성을 위해, 미꾸라지 MAR로부터 ARS를 cloning하여, 그 염기서열을 분석하였다. 총 443 염기들로 구성된 미꾸라지의 ARS는 다른 여러종들의 DNA 복제원점에서 나타나는 것 처럼, AT가 풍부하고, ARS consensus sequences, topoi-somerase II consensus sequences, 그리고 A 흑은 T-box등을 포함하고 있다. 그리고 그 DNA 단편은 복제원점에서 일반적인 양상으로 나타나는 반복적인 inverted sequence들을 가지고 있고, 5개의 가능한 hairpin loop 구조들을 내포하고 있다. 이들 구조는 DNA 복제개시에 관여하는 단백질들의 인지부위로 작용할 것으로 생각된다.

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