Increase in phagocytic activity of peripheal neutrophil and lysozyme activity of blood serum in Korea catfish (Silurus asotus) intraperitoneally injecteded with $\beta$-glucan

$\beta$-glucan을 접종한 한국산 메기(Silurus asotus)의 호중구와 리소자임 활성 증강

  • Park, Sung-Woo (Department of Fish Pathology, College of Ocean Science & Technology, Kunsan National University) ;
  • Kim, Young-Gill (Department of Fish Pathology, College of Ocean Science & Technology, Kunsan National University) ;
  • Choi, Dong-Lim (Kunsan Laboratories, West Sea Fisheries Research Institute, National Fisheries Research & Development Agency)
  • 박성우 (군산대학교 해양산업대학 수족병리학과) ;
  • 김영길 (군산대학교 해양산업대학 수족병리학과) ;
  • 최동림 (국립수산진흥원 서해수산연구소 군산분소)
  • Published : 1996.05.30

Abstract

To examine the effectiveness of $\beta$-glucan in non-specific defence mecanisms of Korean catfish(Silurus asotus), the activities of peripheral neutrophils and serum lysozyme were evaluated after once or twice $\beta$-glucan injections. The number of peripheral neutrophils increased after the injection. The increased number in neutrophils in twice $\beta$-glucan injections maintained longer than in single glucan injecton. But the neutrophils did not showed any remarkable changes in PAS-positive reaction. Peripheral neutrophils from fish injected glucan had enhanced phagocytic activities but there was no significant difference in phagocytic index for the glucan and control groups. An elevation of lysozome activity was also observed in the fish received twice glucan but neither single glucan or physiological saline injection could not evoked an increase of lysozyme activity. These results suggest that $\beta$-glucan can enhance the resistance of Korean catfish against to bacteria infection through the activation of peripheral neutrophils and lysozyme.

한국산 메기(Silurus asotus)에 $\beta$-glucan을 복강내에 접종한 후, 경시적으로 말초혈액중의 호중구의 수와 PAS반응의 변화, 호중구의 탐식능 및 혈청중의 리소자임의 활성변화를 지표로 비특이적방어능력의 증강유무를 조사하였다. 글루칸의 투여후 말초혈액중의 호중구수를 증가시켰으며 투여회수에 따라 2회 접종한 경우 호중구의 증가가 지속되는 기간이 1회 접종어보다 길게 나타났다. 그러나 호중구의 PAS반응에는 변화가 없었다. 또 탐식율도 현저하게 증가하였지만 탐식지수는 변화가 없었다. 리소자임의 활성은 2회 접종시만이 현저히 증가하였으나 1회 접종시에는 활성의 증가가 없었다. 그러므로 $\beta$-glucan의 투여는 말초혈액중의 호중구수와 탐식능의 증가 및 리소자임 활성의 증가등 비특이적방어인자의 활성을 증가시켜 어체를 방어하는 능력을 증강시키는 것임을 알 수 있다.

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Acknowledgement

Supported by : 한국과학재단