Applied Biological Chemistry
- Volume 39 Issue 6
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- Pages.419-423
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- 1996
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Screening of Antimutagenic Activities from Cereals and Beans Including Rice
쌀을 포함한 곡류 및 두류의 항변이원 활성의 검색
- Kang, Mi-Young (Department of Home Economics, Teacher's College, Kyungpook National University) ;
- Choi, Young-Hee (Department of Home Economics, Teacher's College, Kyungpook National University) ;
- Nam, Seok-Hyun (Division of Natural Sciences, College of Natural Sciences, Ajou University)
- Published : 1996.12.31
Abstract
We have established the quantitative method for assay antimutagenic activity from natural products using SOS chromotest technique. Establishment of the method in this study makes it possible to numerize antimutagenic activities from samples in term of the sample amount required for 50% inhibition to mutagenic activity induced by the chemical mutagen under the standard assay condition. Antimutagenic activities of rices from different cultivars as well as other cereals were assayed through this method. The results revealed that antimutagenic activities of mutant cultivar, Suwon 393(Hyangdo) and Sanghaehyanghyulla(Jado), were higher than Chuchung which mainly consumed for steamed rice, and also indicated that antimutagenic activities of cereals, such as job'tear, buckwheat, small red bean, black bean were generally higher than that of brown rice.
SOS chromotest 방법을 이용하여 천연물에 포함된 항변이원성 물질의 활성을 정량화 할 수 있는 항변이원 활성단위 측정법을 개발하였다. 이 방법에 의하여 시료의 항변이원 활성을 특정 화학적 변이원이 유도하는 변이원성이 표준측정 조건하에서 50% 억제되는 시료의 양으로 수치화하는 것이 가능하게 되었다. 확립된 방법으로 쌀의 품종별 항변이원 활성 및 기타 곡류의 항변이원 활성을 조사하였다. 그 결과 변이품종 쌀인 자도와 향도가 취반용으로 사용되는 추청보다 항변이원 활성이 높았고, 일반적으로 검정콩이나 팥과 같은 잡곡의 항변이원 활성이 현미보다 높았다.