Applied Biological Chemistry
- 제39권4호
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- Pages.260-264
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- 1996
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
DNA 입자총에 의한 Cymbidium속 난의 형질전환 조건 검토
Optimization of Cymbidium transformation system by the particle gun techniques
- 홍경애 (제주대학교 방사능이용연구소) ;
- 소인섭 (제주대학교 방사능이용연구소) ;
- 이옥영 (제주대학교 방사능이용연구소) ;
- 정충덕 (제주대학교 방사능이용연구소) ;
- 류기중 (제주대학교 방사능이용연구소) ;
- 유장걸 (제주대학교 방사능이용연구소)
- Hong, Kyung-Ae (Cheju Applied Radioisotope Research Institute, Cheju National University) ;
- So, In-Sup (Cheju Applied Radioisotope Research Institute, Cheju National University) ;
- Lee, Ok-Young (Cheju Applied Radioisotope Research Institute, Cheju National University) ;
- Cheong, Choong-Duk (Cheju Applied Radioisotope Research Institute, Cheju National University) ;
- Riu, Key-Zung (Cheju Applied Radioisotope Research Institute, Cheju National University) ;
- U., Zang-Kual (Cheju Applied Radioisotope Research Institute, Cheju National University)
- 발행 : 1996.08.30
초록
Cymbidium속 난의 형질전환계를 확립하기 위해서, microprojectile bombardment 방법을 이용하여 춘란(Cymbidium virescence)의 rhizome 조직세포에 외래 유전자를 도입하는 조건을 검토하였다. 각 parameter별 적정조건으로서 텅스텐 입자의 크기는
Process of particle bombardment for efficient transformation of Cymbidium virescence rhizome microcross sections was investigated using Biolistic particle delivery system with pBI121 harboring the