Journal of Pharmaceutical Investigation
- Volume 26 Issue 2
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- Pages.83-89
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- 1996
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
Determination of DL-Carnitine Hydrochloride in Pharmaceutical Preparation by HPLC using UV Absorption Derivatives
자외부 흡광 유도체를 이용한 염산 DL-카르니틴의 분석
- Park, Jun-Kyu (Chong Kun Dang Research Institute) ;
- Shin, Hee-Jong (Chong Kun Dang Research Institute) ;
- Kim, Jung-Woo (Chong Kun Dang Research Institute)
- Published : 1996.06.20
Abstract
A reverse-phase HPLC method to determine DL-Carnitine Hydrochloride in pharmaceutical preparation is described. UV absorption derivatives of DL-Carnitine Hydrochloride were formed with p-Bromophenacyl Bromide in an essentially quantitative manner using crown ether as catalyst. The DL-Carnitine-bromophenacyl ester absorbed UV radiation strongly at 254nm, allowing the detection of as small a quantity as 12.5ng of DL-Carnitine Hydrochloride. A linear defection range was