한국전통 식품의 원료인 메주와 누룩에서 분리된 접합균에 대한 연구

Studies on the Fungal Isolates of Mucorales Collected from Korean Home Made Mejus and Nuluks

  • 유기원 (충남대학교 농과대학 식품공학과) ;
  • 성창근 (한국교원대학교 생물과학) ;
  • 이상선 (한국식품개발원 생물공학부) ;
  • 유진영 (한국식품개발원 생물공학부)
  • 발행 : 1996.12.30

초록

The fungal isolates of Mucorales, directly collected from Korean traditional raw materials of Nuruk (raw material for Korean rice wine) and Meju (raw material for Korean soysauces), were compared with those of Rhizopus oryzae purchased. The fungal isolates of Rhizopus, Mucor, and Absidia mostly identified as based on the morphological observations, were evaluated with the PCR-polymorphic bands. The PCR-polymorphic bands of the genomic DNA reacted with the primers of OPD series tenmer were various, but showed averaged 4 to 6 in the agarose-electrophoresis. The dissimilarity coefficient (DC) between two isolates were compared by the cluster analyses, dendrogams and polar ordinations. The isolates of R. oryzae known. showed several groupings within the lower value of DC and were divided to two groups of amylo-process and other fungi with other purposes. The isolates unidentified were identified by the DC made of this results. Taxonomy of these isolates made by the morphological observations were consistent with those resulted above in most case but not in all aspects. More works were needed with the isolates known for detail informations of Mucorales.

키워드

참고문헌

  1. Introductory Mycology Alexopoulos, C.J.;Mims, C.W.;Blackwell, M.
  2. Current Protocols in Molecular Biology Ausubel, F.M.;Brent, R.;Kingston, R.E.;Moore, D.D.;Smith, J.A.;Seidman, J.G.;Struhl, K.
  3. Compendium of soil fungi Domsch, K.H.;Gams, W.;Anderson, T.H.
  4. Science v.252 Recent advances in the polymerase chain reaction Erlich, H.A.;Gelfand, D.H.;Sninsky, J.J.
  5. A manual of soil fungi Gilman, J.C.
  6. Mucorales;The Fungi Hesseltine, C.W.;Ellis, J.J.;Ainsworth, G.C.(ed.);Sparrow, F.K.(ed.);Sussman, A.S.(ed.)
  7. Appl. Microbiol. v.14 Restrctiction fragment length polymorphisms of mitochondrial DNA among Phytophthora capsici isolated from pepper(Capsicum annuum) system Hwang, B.K.;Deck, A.W.;Bhnweg, G.;Prell, H.H.;Heitefuss, R.
  8. J. Bacteriology v.174 Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers Kersulyte, D.;Woods, J.P.;Keath, E.J.;Goldman, W.E.;Berg, E.E.
  9. Korean Mycol. v.21 Identification and Isolation of Zygomyceteous fungi found on Maeju, a Raw Material of korean Traditional soysauces Lee, S.S.;Park, K.H.;Choi, K.J.;Won, S.A.
  10. Korean Mycol. v.23 Meju fermentation for a Raw material of Korean traditional Soy-sauce Lee, S.S.
  11. Statistical Ecology Ludwig, J.A.;Reynolds, J.F.
  12. PCR in situ hybridization; Protocol and application Raven Press Nuovo, G.J.
  13. NTSYS numerical taxonomy system of multivariate statistical program Rohlf, F.;Kishpaugh, J.;Kirk, D.
  14. Theor. Apple. Genet. v.85 Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis Roy, A.N.;Mackay, F.J.;Bousquet, J.
  15. Science v.239 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki, R.K.;Gelfand, D.H.;Stoffel, S.;Scharf, S.J.;Higuchi, R.;Horn, G.T.;Mullis, K.B.;Erlich, H.A.
  16. Science v.230 Enzymatic amplification of ${\beta}$-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Saiki, R.K.;Scharf, S.;Faloona, P.;Mullis, K.B.;Horn, G.T.;Erlich, H.A.;Arnheim, N.
  17. Moleular cloning-A Laboratory manual Sambrook, J.;Fritsch, E.F.;Maniatic, T.
  18. Studies in Mycology v.25 A revision of the genus Rhizopus. II. The Rh. microsporus-group Scripper, M.A.A.;Stalpers, J.A.
  19. Studies in Mycology v.25 A revision of the genus Rhizopus. 1. The Rh. stolonifer-group and Rh. oryzae Scripper, M.A.A.
  20. DNA Fingerprinting in Plants and Fungi Weising, K.;Nybom, H.;Wolff, K.;Meyer, W.
  21. Nucl. Acids Res. v.18 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Williams, J.G.K.;Kubelik, A.R.;Livak, K.J.;Rafalski, J.A.;Tingey, S.V.
  22. Theor. Appl. Genet. v.83 Genetic fingerpfinting of Theobroma clones using randomly amplified polymorphic DNA markers Wkide, J.;Waugh, R.;Powell, W.
  23. Mucorales Zycha, H.;Spieman, P.
  24. 식품미생물학 김찬조;장지현
  25. 전통발효식품의 과학과 연구;전통혼성주의 품질 향상 및 산업화 기술연구 오만진
  26. 전통메주에 관련된 미생물의 생물학적인 분류 및 안정성 연구(위탁-5);전통 장류용 메주의 산업화를 위한 기반기술 연구(전통발효식품의 과학화 연구) 이상선
  27. 한국 농화학학회지 v.13 한국재래식 간장의 발효 미생물에 관한 연구 조덕현;이우진