The Korean Journal of Pharmacology (대한약리학회지)
- Volume 32 Issue 2
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- Pages.243-257
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- 1996
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- 0377-9459(pISSN)
Studies on Intracellular Regulatory Proteins of Pancreatic Exocrine Secretion
이자효소 분비에 관여하는 세포 내 조절 단백에 대한 연구
- Chung, Ku-Yong (Department of Surgery, College of Medicine Ewha Womans University) ;
- Choi, Jae-Won (Department of Pharmacology, Yonsei University College of Medicine) ;
- Choi, Hong-Soon (Department of Pharmacology, Yonsei University College of Medicine) ;
- Kim, Kyung-Hwan (Department of Pharmacology, Yonsei University College of Medicine)
- Published : 1996.09.30
Abstract
CCK and cholinergic agonist stimulate enzyme release from the pancreatic acini via G-protein-mediated activation of phospholipase C, In contrast secretin and related peptides increase the level of cAMP and activate cAMP-dependent protein kinase. Camostat, a synthetic protease inhibitor, causes pancreatic hypertrophy and hyperplasia by increasing the CCK release. In this study, the secretagogue-induced changes of intracellular proteins were examined in the dispersed pancreatic acini of rats with or without camostat treatment. Camostat(FOY-305, 200 mg/kg, p.o.) was given for 4 days twice daily and the dispersed acini were prepared at 12 bouts after last treatment. The profiles of Intracellular phosphoproteins were analyzed by two-dimensional gel electrophoresis after incubating the acini with