The Korean Journal of Pharmacology (대한약리학회지)

The Korean Society of Pharmacology (대한약리학회)
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Effect of Cytokines on the Growth and Differentiation of the Glial Cells from Rat Brain in Culture

랫트 배양 신경교세포의 성장 및 분화에 대한 Cytokine의 효과

  • Kim, Hae-Kyoung (Department of Anesthesiology, School of Medicine, Inha University) ;
  • Youn, Yong-Ha (Department of Phamacology, School of Medicine, Seoul National University) ;
  • Kang, Shin-Chung (Department of Phamacology, School of Medicine, Seoul National University) ;
  • Park, Chan-Woong (Department of Phamacology, School of Medicine, Seoul National University) ;
  • Kim, Yong-Sik (Department of Phamacology, School of Medicine, Seoul National University)
  • 김혜경 (인하대학교 의과대학 마취과학교실) ;
  • 윤용하 (서울대학교 의과대학 약리학교실) ;
  • 강신정 (서울대학교 의과대학 약리학교실) ;
  • 박찬웅 (서울대학교 의과대학 약리학교실) ;
  • 김용식 (서울대학교 의과대학 약리학교실)
  • Published : 1996.09.30
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Abstract

The effects of cytokines on the growth and differentiation of glial cells in culture were evaluated to confirm that cytokines could modify the number and function of glial cells. Proliferation of glial cells was determined by the $^3H-thymidine$ uptake and the double immunostain with anti-cell specific marker and anti-bromodeoxyuridine(BrdU) antibody. To check the effect on the differentiation of glial cells, the amount of glial fibrillar acidic protein(GFAP) and the activity of glutamine synthetase(GS) were measured in astrocytes. And also the amounts of myelin basic protein(MBP) and the activity of 2',3'-cyclic nucleotide phosphohydrolase(CNPase) were measured in oligodendrocytes. Among the cytokines used, only interleukin-$1{\beta}(IL-1{\beta})$ stimulated the growth of type 1 and type 2 astrocyte as well as 0-2A precursor cell. When the functional changes in these glial cells by cytokines were tested, $IL-1{\beta}$ did not increase GFAP content in type 1 and type 2 astrocyte, but $IL-1{\beta}$ increased GS activity in type 1 astrocyte, and slightly decreased this enzyme activity in type 2 astrocyte. Also interleukin-2(IL-2) and $interferon-{\gamma}$ $(IFN-{\gamma})$ inhibited the activity of GS in type 1 and type 2 astrocyte. On the other hand, all cytokines used did not modify the growth and differentiation in oligodendrocytes. From these results we could suggest that $IL-1{\beta}$ increases the growth of type 1 and type 2 astrocyte and also promotes the development for 0-2A precursor cell to type 2 astrocyte.

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