The Korean Journal of Physiology
- 제30권2호
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- Pages.197-208
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- 1996
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- 0300-4015(pISSN)
Isolation and Characterization of Endosome Subpopulation in Chinese Hamster Ovarian Cells
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Suh, Duk-Joon
(Department of Physiology, Dong-A University College of Medicine) ;
- Park, Mi-Yeon (Department of Physiology, Dong-A University College of Medicine) ;
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Jung, Dong-Keun
(Department of Physiology, Dong-A University College of Medicine) ;
- Bae, Hae-Rahn (Department of Physiology, Dong-A University College of Medicine)
- 발행 : 1996.12.30
초록
Endosomes lower their internal pH by an ATP-driven proton pump, which is critical to dissociation of many receptor-ligand complexes, the first step in the intracellular sorting of internalized receptors and ligands. Endosomes are known to exhibit n great range of pH values that can vary between 5.0 and 7.0 within a single cell although the factors that regulate endosomal pH remain uncertain. To evaluate the morphological and topological differences of endosomes in the different stages, confocal microscopy was used. The early endosomes labeled with fluorescein isothiocyanate-dextran for 10 min at
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