BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Specific Labeling of Cytochrome $c_3$ from Desulfovibrio vulgars Miyazaki F and its Assignment

  • Park, Jang-Su (Department of Chemistry, College of Natural Science, Pusan National University) ;
  • Kang, Shin-Won (Department of Chemistry, College of Natural Science, Pusan National University)
  • Received : 1995.04.26
  • Published : 1995.09.30
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Abstract

In order to assign NMR signals, conditions for the specific labeling of cytochrome $c_3$ of D. vulgaris Miyazaki F through the culture in a minimal medium were established. Phenylalanine residue was specifically deuterated at more than 85% efficiency. Cytochrome $c_3$ has two phenylalanine residues. The signals of one phenylalane were missing and this was tentatively assigned to Phe20.

Keywords

References

  1. J. Electroanal. Chem. v.275 Fan, K.;Akutsu, H.;Niki, K.;Higuchi, N.;Kyogoku, Y.
  2. Biochemistry v.29 Fan, K.;Akutsu, H.;Kyogoku, Y.;Niki, K. https://doi.org/10.1021/bi00461a008
  3. J. Mol. Biol. v.172 Higuchi, Y.;Kusunoki, M.;Matsuura, Y.;Yasuoka, N.;Kakudo, M. https://doi.org/10.1016/0022-2836(84)90417-0
  4. Biochim. Biophys. Acta v.497 Matthews, H.R.;Matthews, K.S.;Opella, S.J. https://doi.org/10.1016/0304-4165(77)90134-9
  5. Langmuir v.3 Niki, K.;Kawasaki, Y.;Higuchi, Y.;Yasuoka, N. https://doi.org/10.1021/la00078a018
  6. J. Biomol. NMR v.1 Park, J.S.;Kano, K.;Morimoto, Y.;Higuchi, Y.;Yasuoka, N.;Ogata, M.;Niki, K.;Akutsu, H.
  7. Biochim. Biophys. Acta Park, J.S.;Sagara, T.;Niki, Y.;Kyogoku, Y.;Akutsu, H.
  8. The sulphate-reducing Bacteria Postgate, J.R.
  9. Biochim. Biophys. Acta v.153 Yagi, T.;Honya, M.;Tamiya, T. https://doi.org/10.1016/0005-2728(68)90197-7