BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지

Expression of Cholesteryl Ester Transfer Protein cDNA using Recombinant Vaccinia Viruses

  • Jang, Moon-Kyoo (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Ahn, Byung-Yoon (Department of Genetic Engineering, College of Natural Resources, Korea University) ;
  • Huh, Tae-Lin (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Bok, Song-Hae (Bioproducts Research Group, Genetic Engineering Research Institute, KIST) ;
  • Park, Yong-Bok (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
  • 투고 : 1994.10.24
  • 발행 : 1995.05.31
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초록

cDNA for human cholesteryl ester transfer protein (CETP), a potent atherogenic plasma protein that redistributes the neutral lipids among lipoproteins, was expressed in recombinant vaccinia virus-infected cells (CV-1). Two insertion vectors regulated by different promoters were constructed. The vectors were introduced into human thymidine kinase-negative ($TK^-$) 1438 cells infected with wild-type vaccinia virus (WR strain). Recombinant viruses were selected with 5-bromodeoxyuridine (BUdR) and X-gal and identified with DNA dot blot analysis (vSC11-CETP and vTM1-CETP). The CETP cDNA insert in the recombinant vaccinia virus genome was identified by Southern blot analysis. Transcription of CETP cDNA in CV-1 cells infected with recombinant vaccinia virus was monitored by Northern blot analysis using the CETP cDNA as a probe. Positive signals were detected at 1.8 kb in cells infected with vSC11-CETP and at 2.3 kb in cells infected with vTM1-CETP. The recombinant vaccinia virus-infected CV-1 cells were shown to produce functional CETP when the culture medium was subjected to the CETP assay.

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참고문헌

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