한국양식학회지 (Journal of Aquaculture)
- 제8권1호
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- Pages.1-19
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- 1995
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- 1226-0193(pISSN)
참돔(Pagrus major)의 혈장 난황단백전구체에 대한 효소면역측정법과 난소성숙의 조기판정
Enzymeimmunoassay for the Plasma Vitellogenin and Early Determination of Ovarian Maturation in Red Seabream, Pagrus major
- 한창희 (동의대학교 자연과학대학 생물학과) ;
- 양문호 (국립수산진흥원) ;
- 백재민 (국립수산진흥원) ;
- 임상구 (동의대학교 자연과학대학 생물학과) ;
- 김광현 (동의대학교 자연과학대학 미생물학과)
- Han Chang-Haa (Department of Biology, dongeui University) ;
- Yang Mun-Ho (National Fisheries Research and Development Agency) ;
- Paek Jae-Min (National Fisheries Research and Development Agency) ;
- Lim Sang-Koo (Department of Biology, dongeui University) ;
- Kim Kwang-Hyun (Department of Microbiology, Dongeui University)
- 발행 : 1995.02.01
초록
난황형성 중인 암 참돔의 혈청 내에는 암 특이혈청단백질이 존재하고 있음이 Ouchterlony의 면역확산 검정과 면역 전기영동에 의하여 밝혀졌으며, 이러한 암 특이혈청단백질은 암
In red seabream, Pagrus major the female specific protein in the vitellogenic female serum was identified by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The female specific serum protein might be vitellogenin based on the results of the immunological analysis for the male and vitellogenic female sera and crude egg extracts. Also, it was identified by the immunodiffusion test that the purified yolk protein from ovarian egg extracts has antigenic identities shared with the female specific serum protein. To study the relationship between the maturational stages of gonad and plasma levels of vitellogenin, these were measured from the late resting period (January) to the vitellogenic preiod (April) by the modified enzymeimmunoassay (EIA) using antiserum against yolk protein. The level of plasma vitellogenin began to increase in February (previtellogenesis stage) and continuously increased with the ovarian growth during the vitellogenesis period (March to April). The plasma vitellogenin levels were significantly different between the females and the males in February. Validation for the modified EIA system. was tested .The absorbance curve of serial dilutions of serum from the vitellogenic female was paralleled to the standard curve of yolk protein;
키워드