Journal of Preventive Medicine and Public Health
- Volume 28 Issue 2 Serial No. 50
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- Pages.526-541
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- 1995
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- 1975-8375(pISSN)
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- 2233-4521(eISSN)
Assessment of Validity of RT-PCR and EIA for The Detection of Hepatitis C Virus Infection
C형 간염의 진단을 위한 역전사중합효소연쇄반응과 효소면역측정법의 타당성 평가
- Son, Byung-Chul (Medical college, Inje University) ;
- Chun, Jin-Ho (Medical college, Inje University) ;
- Park, Yeong-Hong (Medical college, Inje University) ;
- Shin, Hai-Rim (Medical college, Dong-A University) ;
- Cho, Kyu-Il (Medical college, Inje University) ;
- Kim, Jong-Han (Graduate School of Public Health, Inje University) ;
- Jung, Kui-Oak (Medical college, Inje University) ;
- Lee, Jong-Tae (Medical college, Inje University) ;
- Lee, Chae-Un (Medical college, Inje University) ;
- Paik, Nak-Whan (Inje University)
- 손병철 (인제대학교 의과대학) ;
- 전진호 (인제대학교 의과대학) ;
- 박영홍 (인제대학교 의과대학) ;
- 신해림 (동아대학교 의과대학) ;
- 조규일 (인제대학교 의과대학) ;
- 김종한 (인제대학교 보건대학원) ;
- 정귀옥 (인제대학교 의과대학) ;
- 이종태 (인제대학교 의과대학) ;
- 이채언 (인제대학교 의과대학) ;
- 백낙환 (인제대학교)
- Published : 1995.06.01
Abstract
This study was conducted to estimate the validity of reverse transcriptase-polymerase chain reaction(RT-PCR) compared to enzyme immunoassay(EIA) for the detection of hepatitis C virus (HCV) infection. EIA for antibody to HCV(anti-HCV) and RT-PCR for HCV was executed on the subjects from Pusan and Kyungnam area with questionnaire survey to collect some relating factors of HCV infection. As the result from 617 cases, the prevalence of HCV infection was 1.5% by EIA and 3.7% by RT-PCR(p<0.05), and the age standardized rate was 1.7% and 3.4% by EIA and RT-PCR, respectively. The prevalence of hepatitis B surface antigen(HBsAg) was 6.8% by enzyme linked immunosorbent assay(ELISA) and the age standardized rate was 7.7%. It was the higher in male group comparing to female group(p<0.01). Both of the prevalence of HCV and HBsAg were higher in elevated asparate aminotransferase(AST) and alanine aminotransferase (ALT) group than in normal AST and ALT group(p<0.01). There was no specific risk factor of HCV infection. Though the degree of agreement of EIA and RT-PCR by gamma statistics was 97.2%, it showed a significant difference between the two methods(p<0.01). For the detection of HCV infection, positive predictive value of EIA was 66.7% and negative predictive value of EIA was 97.2%. This study suggests that negative result to anti-HCV by EIA didn't mean the free state of HCV infection, therefore it would be helpful that further monitoring for HCV infection by RT-PCR in the case of elevated AST and ALT and/or clinically suspected.