한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)
- 제12권2호
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- Pages.121-130
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- 1995
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- 1225-9098(pISSN)
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- 2288-1069(eISSN)
DOI QR Code
해양세균(海洋細菌)을 이용(利用)한 EPA(eicosapentaenoic acid) 생성(生成)에 관한 연구(硏究)
Studies on the EPA(eicosapentaenioc acid) production by marine bacteria
- Joh, Yong-Goe (Department of Food Science and Nutrition, Dong A University) ;
- Kim, Seong-Jin (Department of Food Science and Nutrition, Dong A University) ;
- Kim, Ji-Soo (Department of Food Science and Nutrition, Dong A University) ;
- Lee, Min-Kyeng (Department of Food Science and Nutrition, Dong A University)
- 발행 : 1995.11.30
초록
This project has been worked out for isolation of EPA-producing bacteria from marine source of sea water, sea sediment and intestinal contents eviscerated from some red-muscle fish such as mackerel, horse-mackerel and spike fish. The samples were precultured on the media of PPES-II glucose broth and then pure-cultured on Nutrient agar and P-Y-M glucose. Lipids extracted from those bacterial mass collected by centrifugation were analysed in terms of lipid class and fatty acid composition. The results are resumed as follows : 1. 112 strains from sea water and 76 strains from sea sediment were tested for their EPA producing capability, but both strains of (SA-67 and SA-91) from the former and four strains(SS-35, 37, 51 and 71) from the latter have been proved to produce EPA above the level of 2% of total fatty acids. The strains such as GS-11, 29, 31, HM-9, 29, B-18, 33, 107, YL-129, 156, 203, 77, 104 and 256 which were isolated from fish intestinal contents, have also produced EPA at higher level than 2% of total fatty acids. 2. Contents of total lipids extracted from the cultures of these strains grown at
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