초록
영지 extract가 유당발효성 효모인 Kluyveromyces fragilis KCCM 35458의 발효에 미치는 영향을 조사하기 위하여 탈지유 배양액에 영지 1호의 extract를 0%, 0.1%, 0.5% 및 1.0%의 농도별로 첨가하고 $30^{\circ}C$에서 72시간을 정치배양하면서 조사한 결과는 다음과 같다. 1. 영지 extract를 1.0% 첨가한 시험구(試驗區)는 대조구(對照區)에 비하여 시간이 경과함에 따라 pH에서 빠른 감소를 보였으며 적정산도에서는 빠른 증가를 보였다. 2. $CO_2$ 발생량(發生量)은 영지 extract를 첨가한 전(全) 시험구(試驗區)에서 높았으며 1.0% 첨가구의 효과가 월등하였다. 3. Alcohol 생성량(生成量)은 72시간 배양함에 따라 영지 extract 첨가구(添加區)에서 높게 나타났으며 1.0% 첨가구(添加區)에서 가장 높은 alcohol 생산성을 보였고 발효시간을 단축시켰다. 4. 효모수는 영지 extract 0.5%, 1.0%첨가구에서 대조구에 비하여 현저한 증식효과를 나타내었다. 5. 탈지유 배양액에 있어서의 Kluyveromyces fragilis의 유당발효 상태는 배양시간 경과에 따라 영지 extract 첨가구가 대조구에 비하여 발효가 촉진되었으며 1.0% 영지 extract 첨가구에서 효과적이었다.
In order to investigate the effects of Ganoderma lucidum on the fermentation of media by Kluyveromyces fragilis KCCM 35458, with 0%, 0.1%, 0.5%, and 1.0% (w/v) of Ganoderma lucidum extract were added to skin milk and fermented in the suspension culture at $30^{\circ}C$ for 72 hours. The pH, acidity, $CO_2$ evolution, alcohol production, number of yeast cells and lactose content were investigated. The results are summarized as follows; 1. As fermentation time advanced by the pH was decreased and the acidity was increased in the media of 1.0% Gnaoderma lucidum extract as fermentation time advanced. 2. $CO_2$ evolution was increased in the all of Genoderma lucidum extract and significantly effect showed in 1.0% extract medium. 3. During 72 hours culturing, alcohol was detected in all Gandoderma lucidum extract media, 1.0% Ganoderma lucidum extract medium showed extremly high in alcohol and shortened fermentation time. 4. The number of yeast was increased more at logarithmic phase and the growth of yeast was more rapid in all Ganoderma lucidum extract especially 0.5%, 1.0% media samples than control. 5. As fermentation time goes by, the lactose content of media were decreased more rapidly in 1.0% Ganoderma lucidum extract medium than control.