Journal of Korean Academy of Oral and Maxillofacial Radiology (치과방사선)

Korean Academy of Oral and Maxillofacial Radiology (대한영상치의학회)
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AN EXPERIMENTAL STUDY ON THE RADIOSENSITIVITY AND CHEMOSENSITIVITY OF B16 CELL LINE

B16세포주의 방사선 및 항암제감수성에 관한 실험적 연구

  • Na Seung-Mog (Department of Oral and Maxillofacial Radiology, College of Dentistry, Chonbuk National University) ;
  • Koh Kawng-Joon (Department of Oral and Maxillofacial Radiology, College of Dentistry, Chonbuk National University)
  • 나승목 (전북대학교 치과대학 구강악안면방사선학 교실) ;
  • 고광준 (전북대학교 치과대학 구강악안면방사선학 교실)
  • Published : 1995.12.01
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Abstract

The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. For this study, cell surviving curves were obtained for murine melanoma Bl6 cell line using semiautomated M1T assay. 2,4,6,8, 10Gy were irradiated at a dose rate of 210cGy/min using /sup 60/Co Irradiator ALOORADO 8. After irradiatior, B16 cell lines(2.5×10⁴ cells/ml) were exposed to bleomycin and cisplatin at concentration of 0.2㎍/㎖, 2㎍/㎖ and 20㎍/㎖ for I hour respectively. The viable cells were determined for each radiation dose and/or each concentration of drug. And they were compared to control values. The obtained results were as follows : 1. There was significant difference of surviving fraction at 4, 6, 8, 10Gy on B16 cell line(P<0.05). 2. There was significant difference of cytotoxicity between bleomycin and cisplatin at concentration of 0.2㎍/㎖ and 2㎍/㎖(P<0.05) on B16 cell line, but there was no significant difference of cytotoxicity at concentration of 20㎍/㎖ on B16 cell line. 3. There was significant difference of cytotoxicity of bleomycin after irradiation of 2Gy and 10Gy on B16 cell line(P<0.01). 4. There was significant difference of cytotoxicity of cisplatin at concentration of 20㎍/㎖ after irradiation on B16 cell line.

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