Journal of Microbiology and Biotechnology
- Volume 5 Issue 5
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- Pages.297-301
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Improved Production, and Purification of Aclacinomycin A from Streptomyces lavendofoliae DKRS
- Kim, Wan-Seop (Department of Biology, Korea University) ;
- Youn, Deok-Joong (Central Research Center, Dongkook Pharmaceutical Co.) ;
- Cho, Won-Tae (Central Research Center, Dongkook Pharmaceutical Co.) ;
- Kim, Myung-Kuk (Central Research Center, Dongkook Pharmaceutical Co.) ;
- Kim, Hak-Ryul (Department of Biology, Korea University) ;
- Rhee, Sang-Ki (Korea Research Institute of Bioscience and Biotechnology) ;
- Choi, Eui-Sung (Korea Research Institute of Bioscience and Biotechnology)
- Published : 1995.10.01
Abstract
An anthracycline antibiotic, aclacinomycin A (aclarubicin), was produced from a mutant strain of Streptomyces lavendofoliae. The mutant strain which showed a 4-fold higher productivity of aclacinomycin A compared with the parent strain was also found to produce a significantly higher amount of aclacinomycin A than the reported production strain, Streptomyces galilaeus. The aclacinomycin A was produced up to 125 mg/l using potato starch and soybean meal as carbon and nitrogen sources, respectively, on a 3 liter scale fermentation in a 5 liter jar fermentor. The mutant strain also produced significant amount of aclacinomycins Band Y. Aclacinomycin A was isolated from the culture broth by solvent extractions and further purified by silica gel column chromatography. The yield of aclacinomycin A with over 99